正交试验设计优选新疆紫草的醇提工艺  

Optimization of Alcohol Extraction Technology of Arnebia Euchroma by Orthogonal Design

作　　者：董薇薇[1] 柯木灵 关晶 张建民[3] 

机构地区：[1]安徽中医学院,合肥230031 [2]北京首儿药厂,北京101304 [3]首都儿科研究所,北京100020

出　　处：《中国实验方剂学杂志》2013年第4期48-50,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市科技型中小企业技术创新资金项目(Z09010200260939)

摘　　要：目的:优选新疆紫草中总萘醌的提取工艺。方法:采用紫外-可见分光光度法测定吸光度,以总萘醌提取率为指标,通过正交试验考察提取时间、溶剂用量、提取温度对总萘醌的提取效率的影响。结果:影响新疆紫草中总萘醌提取效果的因素顺序为溶剂用量>提取温度>提取时间。最佳醇提工艺条件为10倍量95%乙醇提取2次,每次1 h,提取温度55℃。结论:该优选的工艺稳定可行,为总萘醌的后期开发提供实验依据。Objective ： To optimize extraction technology of total naphthoquinone from Arnebia euchroma. Method： Absorbance was determined by UV-visible spectrophotometer, with extraction ratio of total naphthoquinone as index, orthogonal test was used to investigate influence of extraction time, solvent consumption and extraction temperature on extraction efficiency of total naphthoquinone. Result： Factors affecting extraction of A. euchroma were： solvent consumption 〉 extraction temperature 〉 extraction time. Optimum extraction technology was as follows： extracted 2 times with 10 times the amount of 95% ethanol at 55 ℃ , 1 h per time. Conclusion： This optimized extraction technology was stable and feasible, it could provide experimental basis for developing of total naphthoquinoe.

关 键 词：新疆紫草 正交试验 提取工艺 总萘醌 

分 类 号：R283.6[医药卫生—中药学]