榅桲叶和果实中总黄酮提取工艺优选  

Optimization of Extraction Technology for Total Flavonoids from Leaves and Fruit of Cydonia oblonga

作　　者：孙晓伟[1] 艾尼瓦尔.吾买尔 周文婷[1] 刘宏炳[2] 田树革[2] 

机构地区：[1]新疆医科大学基础医学院,乌鲁木齐830011 [2]新疆医科大学中医学院,乌鲁木齐830011

出　　处：《中国实验方剂学杂志》2013年第4期68-70,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆维吾尔自治区科技计划项目(201033126);国家自然科学基金(地区基金)项目(81060268)

摘　　要：目的:优选新疆榅桲叶与果实中总黄酮的提取工艺。方法:以总黄酮含量为指标,采用正交试验考察加醇量、超声提取时间、超声功率、乙醇体积分数4个因素对提取工艺的影响。结果:榅桲叶和果实中总黄酮最佳提取工艺均为A3B3C3D1,即加30倍量60%乙醇超声提取40 min,超声功率80%。叶中总黄酮含量较果实中高。结论:优选的总黄酮提取工艺稳定可行,为新疆医药的开发提供实验依据。Objective： To optimize extraction technology of total flavonoids from leaves and fruit of Cydonia oblonga. Method ： With the content of total flavonoids as index, orthogonal design was used to investigate influence of four factors on extraction technology, including the concentration of ethanol, ultrasonic time, ultrasonic power and ethanol amount. Result： Optimum extraction technologies of leaves and fruit were A3B3 CaD1 , ultrasonic extracted 40 min with 30 times the amount of 60% ethanol, ultrasonic power 80% , the content of total flavonoids in leaves was higher than fruit. Conclusion： This optimized technology was stable and feasible, and it could provide experimental basis for development of Chinese medicine in Xinjiang.

关 键 词：楹椁 总黄酮 正交试验 提取工艺 

分 类 号：R283.6[医药卫生—中药学]