藏药川西獐牙菜的质量标准研究  

Quality Control of Traditional Tibetan Medicine Swertia mussotii

作　　者：田成旺[1,2] 张铁军[2] 蒋伶活[1,3] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]天津药物研究院,天津300193 [3]江南大学生物工程学院,江苏无锡214122

出　　处：《中国实验方剂学杂志》2013年第4期75-78,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81001627);天津市科技支撑计划项目(11ZCKFSY01200)

摘　　要：目的:建立川西獐牙莱药材的质量标准。方法:采用薄层色谱法对川西獐牙菜药材进行定性鉴别,采用高效液相色谱法测定主要活性成分獐牙莱苦苷、芒果苷、当药醇苷和齐墩果酸的含量。色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),检测獐芽菜苦苷、芒果苷和当药醇苷的流动相甲醇-0.02%磷酸水(45∶55),检测波长254 nm,检测齐墩果酸的流动相乙腈-甲醇-0.2%冰醋酸水(65∶15∶20),检测波长210 nm,流速1.0 mL.min-1,柱温30℃。结果:川西獐牙菜药材的薄层色谱鉴别特征明显,专属性强;獐牙菜苦苷、芒果苷、当药醇苷和齐墩果酸的HPLC含量测定线性范围分别为0.075～0.75μg(r=0.999 9),0.05～0.5μg(r=0.999 7),0.032～0.32μg(r=0.999 3),0.116～11.6μg(r=0.999 4),平均回收率分别为98.91%(RSD 0.77%),99.61%(RSD 1.38%),100.35%(RSD 0.69%),99.26%(RSD 0.40%),并根据10批药材中相应成分的含量初步制定了其含量限度。结论:川西獐牙菜定性定量测定方法简单准确,能够为川西獐牙菜药材的质量控制提供有效依据。Objective： To establish a method of quality control of Swertia mussotii. Method： TLC method was applied in the qualitative identification of S. mussotii. The content of swertiamarin, mangiferin, swertianolin and oleanolic acid were determined by HPLC equipped with Diamonsil Cls （4.6 mm x 250 mm, 5 μm） column. The mobile phase of swertiamarin, mangiferin and swertianolin consisted of methanol-0.02% phosphoric acid, and detected at the wavelength of 254 nm. The mobile phase of 01eanolic acid consisted of methanol-0.2% glacial acetic acid, and detected at the wavelength of 210 nm. The analysis was done at a flow rate of 1.0 mL min-l. The column temperature was kept at 30 ℃. Result： The linearity of swertiamarin, mangiferin, swertianolin and oleanolic acid was in the range of 0. 075-0.75 μg （ r = o. 999 9 ） , 0.05-0.5 μg （ r = 0.999 7 ） , O. 032-0. 98.91% swertiano accurate 32 μg （r = 0. 999 3） and 0. 116-11.6 μg （r = 0. 999 4） , respectively. The average recoveries were （RSD 0.77% ） for swertiamain, 99.61% （RSD 1.38% ） for mangiferin, 100.35% （RSD 0.69% ） for 1in and 99.26% （ RSD 0.40% ） for oleanolic acid respectively. Conclusion： This method is simple, with good reproducibility. It is suitable for quality control of S. mussotii

关 键 词：川西獐牙菜 獐牙菜苦苷 芒果苷 当药醇苷 齐墩果酸 薄层色谱法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]