根际土壤化学性质对防风色原酮、香豆素含量的影响  

Effects of Rhizosphere Soil Chemical Properties on Content of Chromones and Coumarins in Radix Saposhnikovia Divaricata

作　　者：孙晶波[1,2] 杨鹤[1] 李鑫[2] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]北华大学药学院,吉林吉林132013

出　　处：《中国实验方剂学杂志》2013年第4期88-92,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI03B01)

摘　　要：目的:研究不同时间、不同地点采集的防风根际土壤化学性质对防风色原酮及香豆素含量的影响。方法:甲醇回流提取,HPLC测定防风中8种有效成分的含量;根据国家标准规定方法测定根际土壤pH,速效N,P,K,全N及有机质含量。用SPSS 13.0软件进行相关分析。结果:总体上,1批防风样品中4种色原酮含量高于2批样品,且受其根际土壤影响不显著。2批防风有效成分含量受其根际土壤影响显著,土壤pH与药材中补骨脂素和花椒毒素呈显著负相关(P<0.05),与欧前胡素呈显著正相关(P<0.01);土壤速效P的含量与除升麻素苷之外的7种有效成分均呈显著正相关(P<0.01)。结论:防风色原酮和香豆素类化合物的含量在不同采收期受其根际土壤的影响不同,采集于10中旬的防风有效成分含量受其根际土壤的各种化学性质影响显著。Objective： To study the effects of rhizosphere soil chemical properties on content of chromones and counarins in radix Saposhnikovia divaricata collected from different regions in different harvest times. Method： The HPLC method was adopted for determination content of eight effective components in methanol extracts from radix S. divaricata. According to the National Standard of the People＇ s Republic of China, the pH value, total nitrogen, hydrolysable nitrogen, available phosphorus, potassium and organic matter in rhizosphere soils of S. divaricata were determined. The obtained data were statistical analyzed with SPSS 13.0 software. Result： In general, content of 4 chromones was higher in the first samples than in the second samples and less affected by rhizosphere soils. To the second samples, the soil pH value was significantly negatively correlated to psoralen and xanthotoxin content, but significantly positively correlated to imperatorin content. Whereas, there was a significant positive correlation between soil available phosphorus content and cimicifugin, sec-O-glucosylhamaudol, psoralen, 4＇-O-β-D-glucosyl-5-O-methylvisamminol, xanthotoxin, bergaptol methyl ether and imperatorin content. Conclusion： Rhizosphere soils had different influence on chromones and counarins content in radix S. divaricata collected in different harvest times, especially the samples collected in October.

关 键 词：防风 根际土壤 色原酮 香豆素 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]