HPLC测定大血藤药材中绿原酸的含量  

Determination of Chlorogenic Acid in Sargentodoxa cuneata by High Performance Liquid Chromatography

作　　者：丁越[1] 张永[1] 丁文平[1] 张彤[1] 陶建生[1] 荣蓉[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中国实验方剂学杂志》2013年第4期99-102,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市科委资助项目(09dZ1975500);上海市人才发展基金项目(20111015);上海市教委基金项目(10SG40;J50302)

摘　　要：目的:建立大血藤药材中绿原酸的含量测定方法。方法:采用高效液相色谱法,Diamonsil ODS-C18色谱柱(4.6mm×250 mm,5μm),流动相乙腈-4%磷酸水(11∶89),流速1.0 mL.min-1,检测波长327 nm,柱温25℃。结果:绿原酸在0.000 102～0.010 2 mg线性关系良好。加样回收率为102.4%,RSD 1.7%(n=6)。结论:采用HPLC测定大血藤药材中绿原酸的含量,样品处理方法便捷,测定方法简单,结果准确可靠,可用于大血藤饮片、大血藤制剂的质量控制。Objective： To establish the determination method of chlorogenic acid in Sargentodoxa cuneata. Method： HPLC was used with Diamonsil ODS-Cls column （4.6 mm 250 mm, 5 μm） as chromatographic column and acetonitrile-4% phosphoric acid solution （11：89） as mobile phase. The flow rate was 1.0 mL min-l; the detection wavelength was set at 327 nm and the column temperature was kept at 25 ℃ Result： The linear range of chlorogenic acid was 0. 000 102-0. 010 2 mg. The average recovery was 102.57% , RSD 0. 83% （n = 6）. Conclusion： The method of sample processing was simple and the HPLC method was accurate and reliable. It can be used for the quality control of S. cuneata and its preparation.

关 键 词：绿原酸 高效液相色谱法 含量测定 大血藤 

分 类 号：R284.1[医药卫生—中药学]