诃子草乌配伍与诃子制草乌水煎液中生物碱含量的比较——诃子制草乌炮制原理探讨Ⅱ  

Comparision of Contents of Aconitum Alkaloids between the Decoctions of Aconitum Processed with Myrobalan and Co-decoctions of Aconitum and Myrobalan——Principal of Aconitum Processed with MyrobalanⅡ

作　　者：杨畅[1] 李飞[1] 侯跃飞[1] 刘帅[1] 谭鹏[1] 李向日[1] 杜红[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国实验方剂学杂志》2013年第4期130-132,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京中医药大学自主选题(2011-MZYY-07);中医药行业科研专项项目(201107008);国家自然科学基金项目(81102807)

摘　　要：目的:对生草乌、诃子制草乌水煎液及草乌诃子共煎液中生物碱含量进行比较验证蒙医理论"诃子可解乌头毒"。方法:采用HPLC测定生草乌水煎液、诃子制草乌水煎液及诃子草乌共煎液中新乌头碱、乌头碱、次乌头碱以及苯甲酰新乌头碱、苯甲酰乌头碱、苯甲酰次乌头碱的含量。色谱条件以乙腈-四氢呋喃(25∶15)为流动相A,以0.1 mol.L-1醋酸铵溶液(每1 000 mL加冰醋酸0.5 mL)为流动相B。结果:双酯型生物碱的含量为诃子草乌共煎液>诃子汤制草乌煎液>生草乌煎液;单酯型生物碱的含量为生草乌煎液>诃子草乌共煎液>诃子汤制草乌煎液。结论:诃子可以使草乌中双酯型生物碱缓慢水解,诃子制草乌的毒性低于诃子草乌配伍。Objective： To vertify mongolian medicine theory ＇ myrobalan can decrease the toxicity of aconitum , comparing the contents of aconitum alkaloids among decoctions of aconitum processed with myrobalan, crude aconitum and co-decoctions of aconitum and myrobalan. Method： The HPLC method was used for determination of the contents of aconitine, hypaconitine, mesaconitine, benzoyl aconine, benzoyl hypaconitine and benzoyl mesaconitine in decoctions of aconitum processed with myrobalan, crude aconitum and co-decoctions of aconitum and myrobalan. Result： The contents of diester-diterpene type aeonitum alkaloids during processing in order were co-decoctions of aconitum and myrobalan, decoctions of aconitum processed with myrobalan, decoctions of crude aeonitum. Conclusion： Diester-diterpene type aeonitum alkaloids in aconitum could hydrolysis slowly under the action of myrobalan decoctions, and the toxicity of aconitum processed with myrobalan is lower than the compatibility of aconitum and myrobalan.

关 键 词：诃子 草乌 水煎液 炮制 生物碱 

分 类 号：R284.1[医药卫生—中药学]