HPLC测定苦豆子不同部位中苦豆碱的含量  

Determination of Aloperine in Different Parts of Sophora alopcuroides by HPLC

作　　者：魏蕾初[1] 邓虹珠[1] 张丽[1] 刘振龙 易延逵[1] 

机构地区：[1]南方医科大学中医药学院,广州510515 [2]广东省食品药品检验所,广州510180

出　　处：《中国实验方剂学杂志》2013年第4期140-143,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立苦豆子中苦豆碱的HPLC法;对苦豆子药材不同部位中苦豆碱含量进行考察,确定药材最佳采收部位。方法:采用Kromasil NH2(4.6 mm×250 mm,5μm)色谱柱,以乙腈-无水乙醇-3%磷酸水溶液(70∶15∶15)为流动相,检测波长205 nm,柱温25℃,流速1.0 mL.min-1。结果:苦豆碱的线性范围为11.21～224.20 mg.L-1(r=0.999 9),平均回收率(n=6)98.18%(RSD 4.1%);苦豆子叶、茎中苦豆碱含量分别为0.38%,0.02%,豆荚、花及种子中不含苦豆碱。结论:该方法简便、准确、重复性好,可以作为苦豆子中苦豆碱的含量测定方法;研究结果显示苦豆碱在叶子中含量最高,可以将叶子作为提取分离苦豆碱的最佳药材部位。Objective： To establish a HPLC for determination of aloperine in Sophora alopcuroides and investigate the content of aloperine in different medicinal parts of S. alopcuroides to define an optimum harvest part. Method： The separation was carried out on a Kromasil NH2 column （4.6 mm x 250 mm, 5 μm） ; the mobile phase was acetonitrile-absolute alcohol-3% phosphonic acid water solution （70： 15：15） ; the detective wavelength was set at 205 nm. The column temperature was at kept at 25 C ; the flow rate was 1.0 mL min-1. Result： The linear ranges of aloperine was 11.21-224. 20 mg L-1 （r =0. 999 9） and the average recovery （n =6） was 98.18% （RSD 4, 1%）. The content of aloperine in leaf and stem was 0.38% and 0.02% respectively. However, aloperine was not detected in seed, legume and flower. Conclusion： The method is simple, accurate with good repeatability. It can be used as a method for determination of aloperine in S. alopcuroides ; the results show that leaf has the highest content of aloperine. Hence, leaf is the optimum harvest part for aloperine extraction and separation.

关 键 词：苦豆子 苦豆碱 高效液相色谱法 药用部位 含量测定 

分 类 号：R284.1[医药卫生—中药学]