HPLC测定利咽解毒颗粒中黄芩苷和大黄素含量  

Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC

作　　者：刘阿静[1] 齐广才[1] 刘珍叶[1] 白梓静[1] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《中国实验方剂学杂志》2013年第4期146-149,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定利咽解毒颗粒中黄芩苷和大黄素含量的HPLC法并通过响应曲面优化法对二者的提取工艺进行优化。方法:采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-0.2%磷酸溶液(B)为流动相梯度洗脱,流速1.0 mL.min-1,检测波长为280 nm和245 nm,柱温28℃。结果:黄芩苷和大黄素分别在2.016～134.4,0.408 0～27.20 mg.L-1呈良好的线性关系;平均回收率(n=6)为98.9%和97.8%。结论:方法简便、准确、重复性好,具有较强的实用价值。Objective： To develop an HPLC method for the determination of baicalin and emodin in Liyan Jiedu granules, response surface methodology （RSM） was applied to predict optimum conditions for extraction. Method： The analysis was carried out on an Agilent ZORBAX SB-C18column （4.6 mm x 250 mm, 5 μm）. The mobile phase consisted of aeetonitrile （A） and 0. 2% phosphoric acid （B） with gradient elution at a flow rate of 1.0 mL-min 1. The detection wavelength were set at 280, 245 nm, respectively. The column temperature was 28 C. Result： The linear ranges of baicalin and emodin were 2. 016-134. 4 and 0. 408 0-27.20 mg L-1, respectively. The average recoveries （n = 6） of these were 98.9% and 97.8% , respectively. Conclusion： This method is simple, accurate and repeatable, and can be used for the quality control of Liyan Jiedu granules.

关 键 词：利咽解毒颗粒 黄芩苷 大黄素 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]