清肝利湿解酒汤对酒精性脂肪肝大鼠肝损伤保护作用研究  

Experimental Study on Hepatic-protective Effect of Qinggan Lishi Jiejiu Decoction in Rats with Alcoholic Fatty Liver

作　　者：梁卫[1] 吴承玉[1] 梁涛[1] 

机构地区：[1]南京中医药大学,南京210029

出　　处：《中国实验方剂学杂志》2013年第4期163-167,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部973计划项目(2003CB517101)

摘　　要：目的:研究清肝利湿解酒汤对酒精性脂肪肝(AFL)肝损伤保护作用。方法:将雄性SD大鼠随机分对照组、造模组,采用白酒灌胃、高脂饲料加硫酸亚铁喂食法制备AFL大鼠模型。将造模组随机分为模型组、复方益肝灵片组(0.042 g.kg-1)、清肝利湿解酒汤低、中、高剂量组(10,20,40 g.kg-1),在造模同时,除对照组和模型组给予生理盐水外,其余各组连续灌胃给予相应药物6周,于给药6周末,测定各组大鼠体重、肝指数,采用酶标仪检测血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、谷氨酰转肽酶(GGT)活性、血清和肝组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果:与模型组比较,清肝利湿解酒汤中、高剂量组均显著降低肝指数(3.18±0.42)%,(3.13±0.38)%;降低血清TG(1.09±0.20),(1.04±0.14)mmol.L-1,TC(1.54±0.14),(1.51±0.21)mmol.L-1,LDL-C(0.15±0.02),(0.14±0.01)mmol.L-1;AST(194.10±16.43),(171.50±11.40)U.L-1,ALT(57.70±5.62),(50.80±3.52)U.L-1;GGT(2.80±0.40),(1.80±0.40)U.L-1;MDA(6.50±1.32),(4.70±1.05)μmol.L-1;肝组织MDA(3.07±0.72),(2.07±0.65)nmol.mg-1;显著升高体重(316.47±29.47),(320.71±32.04)g,血清HDL-C(0.55±0.07),(0.56±0.07)mmol.L-1;血清SOD(43.67±2.38),(48.00±1.56)U.mL-1,肝组织SOD(57.31±3.12),(60.67±1.97)U.mg-1。差异均有统计学意义(P<0.05,P<0.01)。结论:清肝利湿解酒汤具有改善肝功能、降低血脂、减轻脂质过氧化反应、增强抗氧化能力的作用。Objective： To investigate the prevention and treatment effects of Qinggan Lishi Jiejiu decoction on rats with alcoholic fatty liver （AFL）. Method： Male SD rats were randomly divided into 2 groups： normal group and model group. Rats were administered with alcohol and fed with high fat diet including FeSO4 to replicate AFL model. All model rats were divided into model group, the compound Yiganling group （0. 042 g ~ kg-1 , ） and the Qinggan Lishi Jiejiu decoction group （ 10, 20, 40 g kg-1 ）. While molding, the normal group and the model group were administered saline, the other groups were given corresponding medicine by garage for 6 weeks. After giving medicine for 6 weeks, we measured the following indexes： weight; liver index; levels of triglyceride （TG） , total cholesterol （TC） , low-density lipoprotein cholesterol （LDL-C）, high-density lipoprotein cholesterol （ HDL-C ）, aspartate aminotransferase （ AST ）, alanine aminotransferase （ ALT ）, glutamyhranspeptidase （GGT） in serum; malondial dehyde （MDA） , superoxide dismutase （SOD） in serum and hepatic tissue. Result： Compared with the model group, in the Qinggan Lishi Jiejiu decoction middle dose group and high dose group, the following indexes were significantly decreased： liver index were （3.18 -＋ 0.42）, （3.13 ±0, 38）% , the contents of TG were （1.09±0.20）, （1.04±0.14） mmol L-1, the contents of TC were （1.54±0.14）, （1.51 ±0.21） mmol.L-1, the contents of LDL-C were （0.15±0.02）, （0.14±0.01） mmol L-1 the contents of AST were （ 194.10 ± 16.43） （ 171.50± 11.40） U L-1 the contents of ALT were （57.70 4.5.62）, （50.80±3.52） U L-1, the contents of GGT were （2.80±0.40）, （1.80±0.40） in serum, the contents of MDA were （6.50±1.32）, （4.70±1.05） I.Lmol＇L-i in serum and （3.07±0.72）, （2.07 ± 0.65） nmol mg-1 in hepatic tissue; the following indexes were significantly increased： body weights were （316.47 4.29, 47） , （3

关 键 词：清肝利湿解酒汤 酒精性脂肪肝 血脂 肝功能 脂质过氧化 

分 类 号：R285.5[医药卫生—中药学]