纳米雄黄对人皮肤鳞状细胞癌A431细胞株增殖抑制及诱导凋亡作用的研究  

Proliferation Inhibition and Apoptosis Induced by Realgar Nanoparticles in Skin Squamous Cell Carcinoma A431 Cells

作　　者：齐元富[1] 李慧杰[2] 聂奔[2] 

机构地区：[1]山东中医药大学附属医院,济南250011 [2]山东中医药大学,济南250014

出　　处：《中国实验方剂学杂志》2013年第4期187-191,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省自然科学基金项目(ZR2010HL050)

摘　　要：目的:探讨纳米雄黄对人皮肤鳞癌A431细胞株增殖抑制及诱导凋亡作用及作用机制。方法:应用MTT比色法、流式细胞仪观察纳米雄黄体对人皮肤鳞状细胞癌A431细胞的增殖及凋亡情况、RT-PCR检测纳米雄黄对生存素(Survivin)mRNA,Caspase-3 mRNA表达的影响。结果:MTT实验及流式细胞仪提示纳米雄黄具有诱导皮肤鳞癌A431细胞凋亡和抑制细胞增殖作用,且随着纳米雄黄剂量的增加作用增强,与顺铂联合应用有协同作用;对照组,5%,10%,20%纳米雄黄组,DDP组,DDP+10%纳米雄黄组的Survivin相对表达量依次为(68.74±1.96)%,(63.93±3.57)%,(57.12±3.93)%,(47.64±6.44)%,(41.44±4.83)%及(32.18±4.10)%;Casepase-3相对表达量依次为(26.26±2.07)%,(32.660±4.42)%,(35.29±5.26)%,(38.52±8.37)%,(45.69±4.19)%及(52.98±6.52)%;纳米雄黄剂量增加可促进Caspase-3的表达,降低Survivin的表达。结论:纳米雄黄可通过诱导肿瘤细胞凋亡和抑制其增殖发挥抗肿瘤作用;其作用机制可能与上调Caspase-3的表达和下调Survivin的表达有关。Objective： To observe effect of realgar nanoparticles （RN） on inhibiting proliferation and inducting apoptosis for human skin squamous cell carcinoma A431 cells, and to explore the related mechanism. Method： MTT colorimetric method and flow cytometric were used to observe the influence of RN on proliferation and apoptosis for human skin squamous cell carcinoma A431 cell, and RT-PCR was used to detect the affect of RN on the expression of survivin and Caspase-3. Result： MTT assay and flow cytometry technique .showed that RN could induce apoptosis in the A431 cells and inhibit their proliferation; and the effects were increased with the dose of RN. The combination of RN with eisplatin showed synergistic effect. In the control group, the 5% , 10% and 20% RN groups, DDP group and DDP ＋10% RN group, survivin relative expression levels were （68.74 ± 1.96）%, （63.93±3.57）%, （57.12±3.93）%, （47.64±6.44）%, （41.44±4.83）% and （32.18± 4.10） % accordingly ; while the Casepase-3 relative expression levels were （26.26 ± 2.07 ） % , （ 32. 660 ±4.42）%, （35.29 ±5.26）%, （38.52 ±8.37）%, （45.69 ±4. 19）% and （52.98 ±6.52）% respectively. RN could increase the expression of Caspase-3 and reduce the expression of survivin. Conclusion： RN may play an anti-tumor effect by inducing tumor cell apoptosis and inhibiting their proliferation; the mechanism may be related to the increase in Caspase-3 expression and the down-regulation of the expression of survivin.

关 键 词：纳米雄黄 人皮肤鳞状细胞癌A431细胞株 凋亡 增殖 

分 类 号：R285.5[医药卫生—中药学]