益智仁挥发油对大鼠快动眼睡眠剥夺恢复后脑组织氨基酸类神经递质含量及其受体表达的影响  

Effects of Volatile Oil Extracted from Alpinia oxyphylla Fructus on Amino Acid Neurotransmitters and its Receptor Expression in Brain of the Model Rats with Sleep Deprivation Recovery

作　　者：崔开宇[1] 王平[1] 游秋云[1] 

机构地区：[1]湖北中医药大学药学院,武汉430065

出　　处：《中国实验方剂学杂志》2013年第4期223-227,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省科技厅2010年研究与开发计划项目(2010BCB004)

摘　　要：目的:观察益智仁挥发油对大鼠快动眼(REM)睡眠剥夺恢复后脑组织氨基酸类神经递质L-谷氨酸(Glu)及γ-氨基丁酸(GABA)含量及其受体表达的影响。方法:将Wistar大鼠随机分为空白对照组(等容生理盐水),REM睡眠剥夺恢复组(等容生理盐水),阳性对照组(安定0.18 g.kg-1.d-1),益智仁挥发油低、高剂量组(9,27 g.kg-1.d-1)。各组灌胃给药2周后,除空白对照组外其余各组用自制改良多平台法剥夺大鼠睡眠48 h制作REM睡眠剥夺模型,睡眠恢复6 h后采用高效液相色谱法检测各组大鼠大脑皮质及下丘脑氨基酸类神经递质Glu及GABA含量变化,采用免疫组化染色和mRNA半定量RT-PCR法检测大脑皮质及海马部位γ-氨基丁酸A受体(GABAAR)α1和γ2亚单位的表达变化,并观察益智仁挥发油对这些指标的影响。结果:与空白对照组比较,REM睡眠剥夺恢复组大鼠大脑皮质及下丘脑Glu含量显著升高,而GABA含量显著降低(P<0.01),大脑皮质及海马部位的GABAARα1和γ2亚单位免疫化学累积吸光度明显较低(P<0.01),皮质部位GABAARα1和γ2mRNA的表达均显著下调(P<0.01),经益智仁挥发油干预后各指标明显趋于正常状态改变(P<0.01,P<0.05)。结论:REM睡眠剥夺恢复可改变皮质及下丘脑部位氨基酸类神经递质Glu,GABA的释放,并使皮质及海马部位GABAARα1和γ2亚单位表达明显下调,益智仁的益智健脑作用机制与其调节神经递质含量及上调大脑皮质及海马部位GABAARα1和γ2亚单位的表达有关。Objective:To observe the effects of volatile oil extracted from Alpinia oxyphylla fructus(VOA) on amino acid neurotransmitters glutamic acid(Glu),gamma-aminobutyric acid(GABA) and its receptor expression in the brain of the model rats with the rapid eye movement(REM) sleep deprivation recovery.Method:Wistar rats were randomly divided into 5 groups: blank control,sleep deprivation recovery,positive control group,sleep deprivation recovery+high and low-dose VOA treatment.After two weeks of administration,model were established with REM sleep deprivation(SD) induced by maded-self multiple platform method(MMPM).Six hours later,the levels of amino acid neurotransmitters Glu and GABA were detected in cortex and hypothalamus of the model rats by HPLC.Using immunohistochemical staining and semi-quantitative analysis of a retrovirus-polymerase chain reaction(RT-PCR),γ-aminobutyric acid A receptor(GABAAR) α1 and γ2 subunit expression were detected in cortical and hippocampal parts of the model rats.What's more,after intervention of VOA,those indicators were studied too.Result: Compared to blank control group,model group rat's content of Glu showed a considerable upper,while GABA showed a considerable down,GABAARα1 and γ2 subunit immunochemistry absorbance accumulated were lower significantly(P<0.01) in cortex and hippocampus,and GABAARα1,γ2 mRNA expression showed lower significantly(P<0.01) in cortex.While those indicators tended to the normal range after the treatment of VOA(P<0.01,P<0.05).Conclusion: REM sleep deprivation recovery could regulate amino acid neurotransmitters and make expression of GABAAR α1 and γ2 subunit markedly down in cerebral cortex and hippocampus.VOA can regulate amino acid neurotransmitters to normal state trend,and upward adjusting GABAAR α1 and γ2 subunit expression in the rat brain.This may be one of the mechanisms for VOA's affect of nourishing the brain and improving intelligence.

关 键 词：REM睡眠剥夺 益智仁挥发油 氨基酸类神经递质 

分 类 号：R285.5[医药卫生—中药学]