枣实蝇不同地理种群的亲缘关系  

Genetic Relation of Carpomya vesuviana from Different Geographiec Populations

作　　者：阿地力.沙塔尔 张伟[3] 程晓甜[2] 朱银飞[2] 骆有庆[1] 陈梦 

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [2]新疆农业大学林学与园艺学院,乌鲁木齐830052 [3]新疆出入境检验检疫局技术中心,乌鲁木齐830083 [4]新疆林业有害生物防治检疫局,乌鲁木齐830000

出　　处：《林业科学》2012年第6期136-140,共5页Scientia Silvae Sinicae

基　　金：新疆维吾尔自治区林业科技专项资金项目"枣树重大检疫性生物灾害应急检疫监控技术研究";自治区科技攻关项目"枣实蝇综合防治及监控研究"(200831108);新疆农业大学前期资助项目(XJAU200911)的部分内容

摘　　要：枣实蝇是一种高度危险性外来有害生物。对吐鲁番市、KSS、HSX和HMS4个不同地区采集的枣实蝇mtDNACOI基因片段测序,并进行序列比较和系统发育分析。结果表明:采自吐鲁番市、HSX、HMS的枣实蝇遗传距离最近,为0.002,其相似性高达99.8%。吐鲁番市枣实蝇和HMS枣实蝇分别与KSS枣实蝇的遗传距离最远,为0.062,其相似性为94.1%。对以上几个地点采集的枣实蝇基因序列与已公开报道的外群蔷薇咔实蝇Carpomya schineriR248FJ571365.1、蔷薇咔实蝇Carpomya schineriR141FJ571364.1、樱桃绕实蝇Rhagoletis cerasiGQ175823.1同源序列进行相似性比较分析,结果显示HMS枣实蝇与蔷薇咔实蝇相似性高达96.5%。根据进化树对比分析,吐鲁番市、HSX和HMS的枣实蝇来自同一地区,而KSS枣实蝇从另一地区传入。Carpomya vesuviana is a high risky exotic pest.In this study,we conducted a test on mtDNACOI Genetic fragments sequencing of C.vesuviana collected from four different regions of Turpan,KSS,HSX and HMS and carried on the sequence comparison and phylogenetic analysis.The results showed that genetic distance of those C.vesuviana which were collected from Turpan,HSX,and HMS was closest with 0.002.Their similarity was up to 99.8 percent.C.vesuviana collected from Turpan and HMS had the longest genetic distance from that collected from KSS,with 0.062,and the similarity was 94.1 percent.A comparison between the above data and the published homologous sequence of C.schineri R248 FJ571365.1,C.schineri R141 FJ571364.1,Rhagoletis cerasi GQ175823.1 showed that the similarity between C.vesuviana from HMS and C.schineri was as high as 96.5 percent.According to the comparison and analysis of the evolutionary tree,C.vesuviana of Turpan,HSX and HMS are inferred originating from the same region,while that of KSS is from other area.

关 键 词：枣实蝇 COI基因 PCR 线粒体DNA 

分 类 号：Q965[生物学—昆虫学]