γ射线降解丝素蛋白的生物相容性研究  

Study on biocompatibility and biodegradation of silk fibroin degraded by γ-rays in vivo

作　　者：金洹宇[1,2] 董朝飞[3] 刘星[1] 刘雨[2,3] 王大朋[1] 宗春燕[1] 张鹤美[1] 赵华[1] 刘建[1] 冯晓青[1] 银华[2,3] 安艳[1,2] 

机构地区：[1]苏州大学医学部公共卫生学院,苏州215123 [2]苏州大学现代丝绸国家工程实验室,苏州215123 [3]苏州大学纺织与服装工程学院,苏州215006

出　　处：《辐射研究与辐射工艺学报》2013年第1期22-25,共4页Journal of Radiation Research and Radiation Processing

基　　金：苏州大学现代丝绸国家工程实验室开放课题(SS115801);江苏省放射医学与防护重点实验室开放课题(KJS0927)资助

摘　　要：丝素蛋白膜,经不同剂量(25、50、100和200 kGy)γ射线照射后植入SD大鼠皮下,植入后第7、14、28、56和84天剖杀取材,进行生化检测。对植入丝素蛋白部位的皮下组织进行病理检查,通过ELISA法检测丝素蛋白植入后的大鼠血清中IL-6和TNF-α含量,取出植入后的丝素蛋白称重,计算质量减少率并绘制质量减少曲线。病理观察及IL-6、TNF-α含量检测结果均提示,丝素蛋白的植入并未引起明显的炎症反应。随着植入时间延长,高剂量组辐照的丝素蛋白质量明显减少。推测γ射线辐照不显著改变丝素蛋白良好生物相容性,可促进丝素蛋白在大鼠体内的降解。Silk fibroin films irradiated by y-rays with different doses （25, 50, 100, and 200 kGy） were implanted subcutaneously into SD rats. On the different dates （7th, 14th, 28m, 56th, and 84th day） after surgery, the rats were given euthanasia and the serum and tissue where the silk fibroin implanted were taken for further pathology tests and the concentration of IL-6 and TNF-a in the serum was also measured. The pathology results and the secretion of IL-6 ＆ TNF-a showed that the silk fibroin did not induce significant immune reactions. The biodegradation rate increased more significantly with the increment of the absorbed doses and implantation time. In conclusion, Y-rays irradiation could increase the biodegradation rate of silk fibroin and almost could not change its biocompatibility.

关 键 词：Γ射线 丝素蛋白 生物相容性 生物降解 

分 类 号：R114[医药卫生—卫生毒理学] R33[医药卫生—公共卫生与预防医学]