核黄素与NADH在紫光波段的激光诱导荧光光谱研究  

Study laser induced fluorescence of riboflavin and NADH in purple band

作　　者：葛琳琳[1] 丁蕾[1] 闫静[1] 王颖萍[1] 郑海洋[1] 方黎[1] 

机构地区：[1]中国科学院安徽光学精密机械研究所环境光谱学研究室,合肥230031

出　　处：《原子与分子物理学报》2013年第1期125-131,共7页Journal of Atomic and Molecular Physics

基　　金：中国科学院合肥物质科学研究院院长基金(Y03AG21136)

摘　　要：基于细胞代谢荧光体的激光诱导荧光探测,在生物反应过程监测与生物活性物质探测中具有广泛的应用.本文利用波长可调谐激光光源,结合液体射流进样装置,研究了核黄素与NADH等生物荧光体在紫光波段(389 nm～404 nm)激发的荧光光谱,并考察了激光强度、样品浓度等参数对荧光光谱特性的影响.实验观察到核黄素的激发光谱在402.5 nm处出现"波谷",具有特征性,选择403.5 nm激光激发,核黄素的浓度灵敏度约为NADH的八百分之一.这些结果为发展生物荧光探测与识别技术提供了新的基础数据.Laser-induced fluorescence measurement based on cell metabolism fluorescent has been applied widely in biological reaction process monitoring and detection of biological active substances. In this pa- per, we use wavelength tunable laser light source combined with liquid jet injection device to study the fluorescence spectroscopy of Riboflavin and NADH excited in purple band （a89 nm^404 nm）, and stud- y the influence of the laser intensity, sample concentration to the fluorescence characteristics. We ob- serve a characteristic ＂valley＂ at 402.5 nm of excitation spectra of Riboflavin. Choosing 403.5 nm exci- tation, riboflavin concentration detection limit is about one eight-hundredth of NADH. These results provide new data base for the development of bioluminescence detection and identification technology.

关 键 词：激光诱导荧光 核黄素 NADH 液体射流 

分 类 号：O561.3[理学—原子与分子物理]