人羊水来源间质干细胞的鉴定及其免疫调节功能和相关机制研究  

The identification of human amniotic fluid-derived mesenchymal stem cell and its immunomodulatory function

作　　者：杜晶春[1] 朱蕊[2] 徐霞[1] 

机构地区：[1]广州医学院第一附属医院检验系,510182 [2]广州医学院第一附属医院妇产科,510120

出　　处：《实用医学杂志》2013年第4期529-532,共4页The Journal of Practical Medicine

基　　金：国家自然科学青年基金项目(编号:81101730);广州医学院第一附属医院院级课题(编号:Y201013)

摘　　要：目的:从人羊水组织中体外培养获得间质干细胞,并探讨其免疫调节能力和相关的调节机制。方法:收集人羊水组织,采用贴壁培养的方法扩增和传代。检测羊水来源的间质干细胞的体外分化能力,并检测其免疫调节能力,同时检测颗粒酶B在人羊水来源间质干细胞中的表达情况,并通过特异性抑制剂阻断方法验证颗粒酶B在其免疫调节中的作用。结果:人羊水来源间质干细胞具有多向分化能力和免疫调节活性,并通过表达颗粒酶B的方式参与其免疫调节功能。结论:体外培养获得了具有免疫调节功能的人羊水来源的间质干细胞,颗粒酶B途径可能是一种新发现的间质干细胞免疫调节方法。Objective To attain human amniotic fluid-derived mesenchymal stem cells （MSCs） by in vitro culture and to explore the possible immunomodulatory mechanism of them. Methods Human amniotic fluid-derived MSCs were collected and amplified by adherent culture, their in vitro differentiation and immunomodulatory function were identified. The expression and immunomodulatory function of Granzyme B in amniotic fluid-derived MSCs were detected. Results The amniotie fluid-derived MSCs have multi-potential differentiation capacity and immunomodulatory function, which was mediated by expressing Granzyme B. Conclusion Human amniotic fluidderived MSCs were successfully obtained. The pathway of Granzyme B may be a new immunomodulatory way of MSCs.

关 键 词：人羊水间质干细胞 体外分化 颗粒酶B 免疫调节 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]