新疆赤芍多糖中糖醛酸含量的测定  

Quantitative determination of uronic acid in Polysaccharides from Radix Paeoniae hybridae

作　　者：王慧[1] 热娜.卡斯木[1] 樊珍珍[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《亚太传统医药》2013年第2期12-14,共3页Asia-Pacific Traditional Medicine

基　　金：新疆维吾尔自治区科技支疆项目(201191259)

摘　　要：目的:测定新疆赤芍多糖中半乳糖醛酸的含量。方法:采用间羟基联苯法对新疆赤芍多糖中的半乳糖醛酸进行含量测定,并研究四硼酸钠-浓硫酸溶液用量、加酸后沸水浴时间、间羟基联苯溶液用量、显色时间及中性糖对测定结果的影响。结果:最佳测定条件为:四硼酸钠-浓硫酸溶液用量5.0mL,沸水浴加热5min,间羟基联苯用量为80μL,显色时间10min,测定波长为525nm。新疆赤芍多糖中糖醛酸的平均含量为24.49%。结论:间羟基联苯法适用于新疆赤芍多糖中半乳糖醛酸的含量测定。Objective：To determine the galacturonic acid content of the Polysaccharides from Radix Paeoniae hybridae. Methods： The method with Meta-hydroxydiphenyl was used. The amount of Na2B4OT-H2SO4, the heating time, m-hydroxydiphenyl, the color development time and the interference by neutral sugar were studied. Results： The optimized parameters were obtained as fol-lows： the dosage of Na2B407-H2SO4 and m-hydroxydiphenyl was 5.0 mL and 80μL, time for heating and color development were 5 min and 10 min respectively. The detection wavelength was 525nm. The average content of galaeturonie acid in Polysaceharides from Radix Paeoniae hybridae is 24.49 %. Conclusion：The method of m-hydroxydiphenyl can be well used for the determination of galacturonic acid in Polysaecharides from Radix Paeoniae hybridae.

关 键 词：新疆赤芍多糖 糖醛酸 间羟基联苯法 

分 类 号：R284.2[医药卫生—中药学]