巴西橡胶树HbNTL1基因启动子的克隆与序列分析  

Cloning and Sequence Analysis of HbNTL1 Promoter from Hevea brasiliensis

作　　者：康桂娟[1] 黎瑜[1] 曾日中[1] 聂智毅[1] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部橡胶树生物学与遗传资源利用重点实验室和省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,儋州571737

出　　处：《生物技术通报》2013年第1期83-88,共6页Biotechnology Bulletin

基　　金：中国热带农业科学院橡胶研究所基本科研业务费专项资金(1630022011024)

摘　　要：膜结合NAC转录因子(NTLs)是植物NAC转录因子家族中一类C端具有跨膜结构域(transmembrane motifs,TMs)的转录调控因子,在植物生长发育、激素调节和逆境胁迫应答中具有重要的功能。根据巴西橡胶树(Hevea brasiliensis)膜结合类NAC转录因子HbNTL1基因cDNA序列,利用基因组步移的方法从巴西橡胶树叶片基因组DNA中克隆获得了HbNTL1基因上游1 718 bp的调控片段。序列分析表明,该段序列含有一个典型的真核生物核心启动子区域,转录起始位点A位于起始密码子上游206 bp处。该启动子序列除了含有多个TATA-box、CAAT-box等基本顺式作用元件外,还存在赤霉素、茉莉酸和脱落酸等激素响应元件以及大量逆境胁迫诱导相关的顺式调控元件,如ABRE、DOFCOREZM、MYBCORE、W-box和MYCCONSENSUSATHSE等反应元件,表明HbNTL1转录因子可能是一个逆境胁迫相关NAC转录因子,在橡胶树抵御逆境胁迫的生理过程中具有重要功能。Membrane-bound NAC transcription factors （ NTLs ） are a group of NAC transcription factors with transmembrane motif （ TMs ） in the C-terminal region, play important roles in plant growth and development, hormone regulations and stress responses. A 1 718 bp 5＇ regulatory sequence of HbNTL1 was cloned from leaf genomic DNA of H. brasiliensis based on the cDNA sequences using Genome Walking method. Sequence analysis showed that it consisted of a typical core promoter region of eukaryotic, and the transcription start site （ TSS, A ） located 206 bp upstream of the initiation codon ATG. The promoter contains the basic elements ： TATA-box, CAAT-box, and some hormone responsive elements as well as and multiple stress-induced elements, such as, ABRE, DOFCOREZM, MYBCORE, MYCCONSENSUSATHSE and W-box. It is suggested that HbNTL1 gene may play important roles ir~ response to various stresses in rubber tree as a stress-related NAC transcription factor.

关 键 词：巴西橡胶树 HbNTL1启动子 顺式调控元件 逆境胁迫 

分 类 号：S794.1[农业科学—林木遗传育种]