转基因水稻华恢1号检测用质粒标准分子研制  

Development of a Reference Plasmid for the Quantitative Detection of Genetically Modified Rice Huahui 1

作　　者：李亮[1] 王晶[1] 臧超[1] 隋志伟[1] 赵正宜[1] 

机构地区：[1]中国计量科学研究院,北京100013

出　　处：《生物技术通报》2013年第1期96-101,共6页Biotechnology Bulletin

基　　金：国家科技支撑计划项目课题(2008BAK41B01)

摘　　要：质粒标准分子作为阳性标准品已广泛用于转基因作物及产品的核酸定量检测。但是目前多数质粒标准分子只用于研究,未能成为国家级标准物质。因此,按照国家一级标准物质技术规范,研制了用于检测转基因水稻华恢1号的质粒标准分子,内容包括质粒分子的构建、纯度分析、特异性检测、可替代性研究、均匀性检验、稳定性考察、定值与不确定度评估。结果表明,该质粒分子标准物质半年内稳定,可替代基因组作为华恢1号定量的阳性标准。With the development of genetically modified organism （ GMO ） detection techniques, the reference plasmids have been the most effective and important materials for GMO quantification as a positive control. But many of them are used in researching, not developed a certified reference material. Here, we outline a reference plasmid, developed according the Technical Norm of Primary Refernce Material （ JJF 1006-1994 ） , including preparation, analysis of purify, homogeneity, stability, commutability, eharacterisation and uncertainty. Results suggested the reference molecule is stable for 6 months and particularly suitable for the rapid identification of GMO components in unknown samples and can reduce cross contamination

关 键 词：华恢1号 转基因水稻 实时荧光定量PCR 标准分子 

分 类 号：S511[农业科学—作物学]