甘蓝型油菜高效离体再生体系的建立  

Establishment of Effective Regeneration System of Brassica napus L.in vitro

作　　者：杨长友[1] 袁中厚[1] 郑小敏[1] 张涛[1] 

机构地区：[1]重庆师范大学生命科学学院,重庆401331

出　　处：《生物技术通报》2013年第1期111-115,共5页Biotechnology Bulletin

基　　金：国家自然科学基金(31171588);重庆市自然科学基金(cstc2010BB5324;cstc2012jjA80010);重庆市教委科技项目(KJ100604)

摘　　要：以甘蓝型油菜(Brassica napusL.)HC8为材料,从无菌苗苗龄、预培养基激素浓度、预培养天数、6-BA及NAA的浓度等方面对影响油菜组织培养的因素进行了分析研究,建立了甘蓝型油菜品系HC8的离体再生技术体系。结果表明,该油菜组织培养的最佳苗龄为5 d;最佳预培养时间为5 d,最佳2,4-D浓度为1.0 mg/L;子叶最佳诱导培养基为MS+2.0 mg/L 6-BA+0.05 mg/LNAA+3.5 mg/L AgNO3或MS+3.0 mg/L 6-BA+0.1 mg/L NAA+3.5 mg/L AgNO3,该条件下子叶愈伤组织诱导率最高可达100%,再生频率及分化频率分别可达88.0%和108.33%;下胚轴最佳诱导培养基为MS+4.0 mg/L 6-BA+0.05 mg/L NAA+3.5 mg/L AgNO3,子叶愈伤组织诱导率最高可达95.24%,再生频率及分化频率分别可达81.82%和104.55%;最佳生根培养基为MS+0.5 mg/L NAA,生根率最高为90.0%。We examined the effect of the seedling age of sterile seedling, the days and concentrations of growth regulators in pre- cultivation, the concentrations of 6-BA and NAA on rape tissue culture with HC$ （ Brassica napus L. ） . We established the technical system of regeneration of HC8 in vitro with high frequency. The results showed that the best seedling age and the optimal day of pre-cultivation was five days for rape tissue culture with HC8, and the optimal concentration of 2, 4-D was 1.0 mg/L. The best medium for callus induction and differentiation of cotyledons were MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA＋3.5 mg/L AgNO3 or MS＋3.0 mg/L 6-BA＋0.1 mg/L NAA＋3.5 mg/L AgNO3. With the medium, the ratios of the highest level of callus induction, regeneration and differentiation were 100%, 88% and 108.33%, respectively. The best medium for callus induction and differentiation of hypocotyls were MS＋4.0 mg/L 6-BA＋0.05 mg/L NAA＋3.5 mg/L AgNO3. With the medium, the ratios of the highest level of callus induction,＂ regeneration and differentiation were 95.24%, 81.82% and 104.55%, respectively. The best medium for rooting of regenerated plant was MS basal medium supplemented with 0.5 mg/L NAA with the ratio of the highest level of rooting （ 90% ）.

关 键 词：甘蓝型油菜 愈伤组织诱导 离体再生 芽分化 

分 类 号：S565.4[农业科学—作物学]