双元细菌人工染色体基因组文库研究进展  

Research Progress on Binary Bacterial Artificial Chromosome Genomic Library

作　　者：朱丹[1,2] 张杰伟[2] 陈亚娟[2] 张少斌[1] 魏建华[2] 

机构地区：[1]沈阳农业大学,辽宁沈阳110866 [2]北京市农林科学院北京农业生物技术研究中心

出　　处：《现代农业科技》2013年第2期29-30,36,共3页Modern Agricultural Science and Technology

基　　金：国家自然科学基金(30972392);"十二五"863计划(2011AA100201)

摘　　要：酵母人工染色体(YAC)、细菌人工染色体(BAC)是伴随着人类基因组计划的实施而产生的,这些大片段基因克隆载体的应用推动了分子生物学、遗传学等学科的快速发展。以农杆菌介导的直接转化植物为代表的双元细菌人工染色体(BIBAC)载体的发展,加速植物基因组的研究,成为植物基因组分析、基因功能鉴定、染色体结构与功能关系研究的重要工具。介绍了人工染色体文库的发展史,根据建库经验,着重阐述了BIBAC文库的构建、鉴定与应用。Cloning vector YAC,BAC produce with the human genome project appearing.These large fragment gene cloning vector application promoted molecular biology,genetics and other scientific development.According to agro-bacterium mediated plant transformation vector BIBAC development,the vector BIBAC accelerate plant genome research,become important tools of genome analysis and functional identification of genes,chromosome structure and function relationship.This paper introduced the history of artificial chromosome briefly,then the construction,identification and application of BIBAC library were described in detail based on the experiences of authors.

关 键 词：人工染色体 双元细菌人工染色体文库 基因组文库 

分 类 号：Q343.1[生物学—遗传学]