体外溶血评价方法中最佳离心转速和离心时间的考察  

Study on the optimal centrifugal velocity and time in evaluation of hemolysis in vitro

作　　者：吕雅婷[1] 丁选胜[1] 孙露[1] 唐文婧[1] 

机构地区：[1]中国药科大学药学院,江苏南京210009

出　　处：《安徽医药》2012年第10期1402-1405,共4页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(No 81073145)

摘　　要：目的确定最佳的离心转速和离心时间,保证体外溶血评价结果的准确性。方法选择不同离心转速(750、1 000、1 250、1 500、1 750和2 000 r.min-1),每个离心转速分别考察不同离心时间(5、10、15和20 min)。各组离心后于545 nm下测定吸光值并进行红细胞计数。结果离心转速为1 500 r.min-1,离心时间为15 min时,红细胞达到最大下沉且对红细胞的损伤最小。结论离心转速为1 500 r.min-1,离心时间为15 min可作为体外溶血评价方法中的最佳离心转速和离心时间。Objective To determine the optimal centrifugal velocity and time so as to ensure the accuracy of evaluation of hemolysis in vitro.Methods Different centrifugal velocities were selected including 750,1 000,1 250,1 500,1 750 and 2 000 r·min-1.Each of centrifugal velocity was correspondent to four kinds of centrifugal time such as 5,10,15 and 20 min.After centrifugalization,absorption value and red blood cell count were measured at 545 nm in all groups.Results When the centrifugal velocity was 1 500 r·min-1 and the time was 15 min,the maximum subsidence achieved and red blood cell injury was slightest under this condition compared with other groups.Conclusion So,1 500 r·min-1 and 15 min can be defined as the optimal centrifugal velocity and time in the evaluation of hemolysis in vitro.

关 键 词：体外溶血 评价方法 离心转速 离心时间 

分 类 号：R446.1[医药卫生—诊断学]