HPLC法测定缬沙坦氨氯地平片含量  

Determination of valsartan and amlodipine in valsartan and amlodipine tablets by HPLC

作　　者：王康蕊[1] 严相平[1] 谢浩[1] 

机构地区：[1]南京工业大学江苏省药物研究所,江苏南京210009

出　　处：《安徽医药》2012年第10期1432-1433,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立缬沙坦氨氯地平片含量测定的方法。方法采用Waters Symmetry C18(3.9 mm×150 mm,5μm),以乙腈∶甲醇∶三乙胺溶液[磷酸调pH(4.3±0.1)]15∶35∶50为流动相,流速1.0 ml.min-1,检测波长237 nm,柱温30℃。以流动相配制对照品及供试品溶液,测定浓度为氨氯地平5 mg.L-1,缬沙坦160 mg.L-1。结果缬沙坦在128.32～192.48 mg.L-1范围内线性良好,n=5,r=0.999 7,氨氯地平在4.07～6.14 mg.L-1范围内线性良好,n=5,r=0.999 3。缬沙坦和氨氯地平平均回收率分别为99.93%、99.85%,RSD为0.30%、0.34%。结论该方法简便快速,灵敏度高,准确可靠。Objective To establish an HPLC method for the determination of valsartan and amlodipine in valsartanand amlodipine Tablets.Methods Waters symmetry C18（3.9 mm×150 mm,5 μm）was used.The mobile phase was composed of acetonitrile∶ methanol∶ triethylamine solution（adjusted with phosphoric acid to a pH of 4.3±0.1） 15∶ 35∶ 50.The flow rate was 1.0 ml·min-1 and detection wavelength 237 nm.The temperacture was 30℃.The sample solution concentrations were amlodipine 5 mg·L-1,valsartan 160 mg·L-1.Results Valsartan concentration achieved good linearity in the range of 128.32~192.48 mg·L-1,n =5,r=0.999 7,amLodipine in the range between 2.00 and 12.00 mg·L-1,n=5,r=0.999 3.The average recoveries of valsartan and amlodipine were 99.93%,99.85%,and RSD were 0.30% and 0.34%.Conclusion This assay is simple,rapid,sensitive,accurate and reliable.

关 键 词：缬沙坦 氨氯地平 含量测定 HPLC 

分 类 号：R927[医药卫生—药学]