脐血MSCs移植对急性心肌梗死大鼠心肌的修复作用  

Umbilical cord blood MSCs transplantation myocardial repair of acute myocardial infarction in rats

作　　者：龚敏[1] 李涛[1] 雷靖祎[1] 辛渭川[1] 

机构地区：[1]西安市中心医院心内科,陕西西安710061

出　　处：《江苏大学学报（医学版）》2012年第5期369-373,共5页Journal of Jiangsu University：Medicine Edition

基　　金：陕西省科学技术研究发展项目(2006k13-G2)

摘　　要：目的:观察体内心肌缺血微环境诱导下人脐血间充质干细胞(umbilical cord blood mesenchymal stem cells,UCBMSCs)向心肌样细胞的定向分化及对急性心肌梗死(acute myocardial infarction,AMI)大鼠心功能、新生血管的影响。方法:收集健康产妇脐血细胞,采用密度梯度离心结合差速贴壁的方法分离MSCs;健康成年SD大鼠30只,制备成AMI大鼠模型后分为2组,移植组在心肌梗死周边区注射移植GFP标记的UCBMSCs;对照组在心肌梗死周边区注射等量生理盐水。术后4周行超声心动图检测及血流动力学检查,并取心脏组织行冰冻切片,免疫荧光染色检测UCBMSCs心肌特异性蛋白cTnT和Connexin43的表达;并进行抗Ⅷ因子抗体免疫组化染色,观察心肌毛细血管密度(myocardial capillary density,MCD)的变化。结果:与对照组相比,术后4周移植的UCBMSCs表达心肌特异性蛋白cT-nT和Connexin43,对照组无心肌特异蛋白表达;移植组LVEDD、LVESD明显减小,而LVEF、LVFS明显增加,血流动力学指标明显改善;免疫组化染色结果显示,移植组梗死周边区MCD较对照组明显增加,移植组为(4.16±0.2)个/HP(×400),对照组为(2.29±0.3)个/HP,两组比较差异有统计学意义(P<0.01)。结论:UCBMSCs移植可在大鼠AMI部位存活,并向心肌样细胞分化;UCBMSCs移植后明显改善AMI大鼠心功能及心室重构,促进毛细血管新生。Objective： To observe the induction of myocardial ischemia micro-environment in vivo, and how human umbilical cord blood mesenchymal stem ceils （UCBMSCs） differentiate directly to myocardial- like cells, and their effect on the heart function and revascularization in acute myocardial infarction （AMI） rat and to explore their possible mechanism. Methods： Collected health maternal cord blood cells; density gradient centrifugation and differential speed adherent was used to isolate MSCs. Thirty healthy adult SD rats was preparated to establish rat AMI model. They were divided into 2 groups： transplantation group was peripheral injected of transplanted GFP-labeled UCBMSCs, and control group injected of normal saline. Four weeks later, echocardiography figure detection and hemodynamic check was used, and heart tissue fro- zen section was harvested to be tested by immunofluorescence staining cbMSCs myocardial specificity protein cTnT and Connexin43 expression; and anti-Vlll immunohistochemical staining, myocardial capillary density （MCD） as well. Results： Compared with the control group, 4 weeks after transplantation UCBMSCs expres- sion of cardiac-specific proteins cTnT Connexin43, the control group had no cardiac-specific protein expres- sion; transplantation group LVEDD, LVESD significantly reduced LVEF, LVFS increased significantly,and the hemodynamic indicators improved significantly; immuno-histochemical staining showed that the trans- plantation group infarct border zone MCD increased significantly compared with the control group, the trans- plantation group（4.16 ± 0.2）/HP（ ×400）, and the control group（2.29±0.3）/HP; difference between the two groups was significant （P 〈 0.01 ） Conclusion： UCBMSCs transplantation was survival after rats AMIin their infarct parts and cardiomyocyte-like cell differentiated; UCBMSCs transplantation significantly im- proved AMI cardiac function and ventricular remodeling, and promoted angiogenesis.

关 键 词：心肌梗死 脐血干细胞移植 心肌样细胞 血管新生 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]