基于rDNAITS和beta-tubulin gene部分序列分析灵芝属菌株的遗传关系  

Assemblage of 38 Ganoderma Strains Based on Combined rDNA ITS and Partial β-Tubulin Gene Sequences

作　　者：唐传红[1] 苏春丽[2] 张劲松[1] 杨焱[1] 唐庆九[1] 贾薇[1] 冯娜[1] 刘艳芳[1] 吴迪[1] 

机构地区：[1]上海市农业科学院食用菌研究所、农业部南方食用菌资源利用重点实验室、国家食用菌工程技术研究中心、国家食用菌加工技术研发分中心、上海市农业遗传育种重点开放实验室,上海201403 [2]成都市食品药品检测中心,成都四川610031

出　　处：《食用菌学报》2012年第3期37-41,共5页Acta Edulis Fungi

基　　金：科技部农业科技成果转化资金(编号:2012GB2C000148)的部分研究内容

摘　　要：联合ITS序列和beta-tubulin基因部分序列的系统发育分析将分属于8个种的38株菌株分为9个聚类群,聚类结果与物种结果基本一致,只有少数几个菌株,如GL81菌株聚类结果与拉丁名不符,可能由于来源地给出的定名不够准确。根据结果联合信息分析可以对收集的不同菌株进行种类的初步分析。Thirty-eight fungal strains, representing eight species of Ganoderma, obtained from Chinese sources and elsewhere, were separated into nine groups based on an analysis of combined rDNA ITS and partial β-tubulin gene sequences. The delineated groups were coincident with taxonomic species assignments except in the case of strains GLll and GL81, which had been identified as G. lucidum but exhibited closer similarity with G. oerstedii. Our data suggested this methodology can be used for the preliminary assignment of newly isolated Ganoderma strains.

关 键 词：灵芝 ITS beta-tubulin基因 遗传多样性 

分 类 号：S567.31[农业科学—中草药栽培]