二次酶解法提取硫酸软骨素的工艺优化研究  

Optimization of Chondroitin Sulfate Extraction by Twice Enzymolysis

作　　者：陈亚[1] 高盐生[1] 

机构地区：[1]江苏省盐城技师学院,江苏省滩涂生物资源与环境保护重点建设实验室,江苏盐城224002

出　　处：《食品研究与开发》2012年第12期69-72,共4页Food Research and Development

基　　金：江苏省滩涂生物资源与环境保护重点建设实验室开放课题项目(JLCBE11006)

摘　　要：以猪喉软骨为原料,在碱液提取的基础上,采用胰酶和中性蛋白酶二次酶解的方法提取硫酸软骨素,并对其中的第二次酶解工艺进行优化研究。结果表明,第二次酶解的最佳工艺组合为,酶浓度1.1%、酶解温度44℃、pH7.0、酶解时间2.0 h。采用上述组合,以氨基葡萄糖含量为纯度指标,硫酸软骨素中氨基葡萄糖含量达33.59%,相对于一次酶解,氨基葡萄糖含量提高了7.65%。研究结果具有工业应用价值。The chondroitin sulfate was extracted from pig laryngeal cartilage.Based on alkali extraction,twice enzymolysis of trypsin and neutral protease was used and the technique of the second enzymolysis was improved.The results indicated that the best combination of extracting technique of the second enzymolysis was as follows.the enzyme concentration was 1.1 %,the temperature was 44 ℃,pH value was 7.0,and the time was 2.0 h.The content of glucosamine in chondroitin sulfate was 33.59 % after the optimization of the technique.The content of glucosamine was 7.65 % higher than that of the enzymolysis only one time.The results can be calculated for industry use.

关 键 词：二次酶解 工艺优化 硫酸软骨素 猪喉软骨 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]