致命鹅膏菌肽类毒素的体外抗肿瘤作用  

Purification of Toxins from Amanita exitialis and Their Anti-tumor Effects in vitro

作　　者：邓旺秋[1] 肖正端[1] 李鹏[1] 李泰辉[1] 

机构地区：[1]广东省微生物研究所、广东省菌种保藏与应用重点实验室、广东省微生物应用新技术公共实验室、广东省华南应用微生物重点实验室、省部共建国家重点实验室培育基地,广东广州510070

出　　处：《食用菌学报》2012年第4期71-78,共8页Acta Edulis Fungi

基　　金：国家自然科学基金项目(编号:31101592);广东省科技计划项目(编号:2009B030803011);广东省自然科学基金项目(编号:E05202480)的部分研究内容~~

摘　　要：采用噻唑蓝(MTT)法对从致命鹅膏(Amanita exitialis)子实体中获得的α-鹅膏毒肽、β-鹅膏毒肽、三羟鹅膏毒肽酰胺、羧基一羟鬼笔毒肽、羧基二羟鬼笔毒肽、羧基三羟鬼笔毒肽和脱氧二羟毒伞素和一种未知毒素共8个纯的毒素化合物进行体外抗肿瘤活性研究。结果表明:8种鹅膏毒素对人宫颈癌细胞株Hela和肝癌细胞株7402的生长均有抑制作用,其中α-鹅膏毒肽和β-鹅膏毒肽具有较强的抑制活性。这两种毒素浓度100μg/mL、作用72h时抑制率达60%以上,与阳性对照5-氟脲嘧啶的抑制效果相近,但对肝正常细胞L02的增殖抑制仅为对照的50%。Eight toxins,isolated and purified from fruit bodies of Amanita exitialis using high performance liquid chromatography,consisted of α-amanitin,β-amanitin,amaninamide,phallacin,phallacidin, phallisacin,desoxoviroidin and an unknown toxin.Evaluation of the in vitro anti-tumor activities of those toxins,determined using the MTT assay,showed that all the toxins exerted inhibitory effects on the proliferation of Hela and liver cancer(7402)cells.α-Amanitin andβ-amanitin strongly inhibited(>60%) Hela and 7402cell growth at 100μg/mL concentrations after 72hexposure,only slightly less(～7%)than the inhibition rate recorded with 5-fluorouracil(5-Fu)(positive control).However,under the same test conditions,normal liver L02cell proliferation was inhibited～40%by both toxins,compared with 81%by 5Fu.Our data indicated that amanita toxins have considerable potential for use in the treatment of certain tumors.

关 键 词：鹅膏毒素 肿瘤细胞 抑制增殖 

分 类 号：R285[医药卫生—中药学]