慢病毒介导的水通道蛋白8短发卡RNA抑制SiHa人子宫颈癌细胞的迁移研究  

Aquaporin 8 short hairpin RNA mediated by lentivirus inhibits the migration of SiHa human cervical cancer cells

作　　者：史永华[1] 尼加提.热合木 塔拉甫.托坎 拉莱.苏祖克 

机构地区：[1]新疆医科大学基础医学院病理教研室,新疆乌鲁木齐830011

出　　处：《中国实用妇科与产科杂志》2013年第2期123-127,共5页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金地区基金(81060216);新疆医科大学科研创新基金(XJC201011)

摘　　要：目的探讨慢病毒介导的AQP8短发卡RNA(short hairpin RNA,shRNA)对SiHa人子宫颈癌细胞迁移的影响。方法 2011年6月构建慢病毒介导的AQP8 shRNA稳定转染SiHa细胞系,新疆医科大学基础医学院病理教研室通过Transwell侵袭和迁移实验、损伤愈合实验及增殖和贴附能力实验检测AQP8基因沉默对SiHa细胞迁移的抑制作用。结果 (1)成功构建慢病毒介导的AQP8 shRNA稳定转染SiHa细胞系;(2)AQP8shRNA-SiHa侵袭迁移率明显低于对照组(F=196.451,P<0.05);AQP8shRNA-SiHa愈合速度明显低于对照组(F=37.725,P<0.05);AQP8shRNA-SiHa组增殖及基质贴附能力与对照组相比差异均无统计学意义(P>0.05)。结论抑制AQP8基因表达可以抑制SiHa细胞的迁移和潜在的侵袭过程。Objective To probe the influence of AQP8 short hairpin RNA （shRNA） mediated by lentivirus on migration of SiHa human cervical cancer cells. Method To build stable transfection SiHa cell line of AQP8shRNA mediated by lentivirus, furtherly to detect inhibitory effect of AQP8 gene silence on migration of SiHa cells by transwell migration and invasion assays, wound healing assay and tumor cell growth, substrate adherence assays. Result （ 1 ） To successfully build stable transfection SiHa cell line of AQP8shRNA mediated by lentivirus; （2）Migration and invasion rate of AQP8shRNA-SiHa was obviously lower than control groups （P 〈 0.05 ） ;Wound healing speed of AQP8shRNA-SiHa was obviously lower than control groups （P 〈 0. 05 ） ;Difference of growth and substrate adherence between AQP8shRNA-SiHa and control groups was not significant （P 〉 0. 05 ）. Conclusions Silence of AQP8 gene expression can inhibit migration of SiHa cells and potential invasive process.

关 键 词：慢病毒 AQP8基因抑制 SIHA 肿瘤细胞迁移 

分 类 号：R711.74[医药卫生—妇产科学]