β-arrestin2在子宫内膜癌中的表达及其与血管生成的关系  

The expression of β-arrestin 2 in endometrial carcinoma and its relationship with angiogenesis

作　　者：黄强[1] 孙秀丽[1] 王建六[1] 魏丽惠[1] 

机构地区：[1]北京大学人民医院妇科,北京100044

出　　处：《中国实用妇科与产科杂志》2013年第2期132-134,共3页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金(810293)

摘　　要：目的探讨β-arrestin 2在不同子宫内膜组织中的表达及其与血管生成之间的关系。方法选取2002年9月至2010年12月间北京大学人民医院的组织标本75例,其中正常子宫内膜19例,子宫内膜非典型增生20例,子宫内膜癌24例,复发转癌12例。对其进行CD34及β-arrestin 2免疫组化染色。分别转染β-arrestin 2空白荧光质粒、全长质粒及干扰RNA质粒入子宫内膜癌HEC-1A细胞,ELISA方法测定各组上清中VEGF的表达量。结果子宫内膜癌组微血管密度(MVD)值明显高于正常内膜组及非典型增生组(P<0.05);子宫内膜癌组中β-arrestin 2表达明显高于其他各组(P<0.001);MVD与β-arrestin 2表达呈正相关(r=0.557,P<0.001);过表达β-arrestin 2可促进VEGF分泌。结论β-arrestin 2可促进子宫内膜癌细胞分泌VEGF,从而促进新生血管的形成,导致子宫内膜癌的进展。Objective To identify the difference of β-arrestin 2 expression between normal endometrium, atypical endom-etrial hyperplasia and endometrial cancer and its relationship with tumor angiogenesis. Methods （1） Seventy-five par-affin uterine specimens were collected from Peking University People＇s hospital from September, 2002 to December, 2010. There were 19 cases with normal endometrium, 20 cases with atypical endometrial hyperplasia and 36 cases with endometrial cancer, among which, there were 12 cases with metastases. CD34 and β-arrestin 2 were detected with immu-nohistochemistry staining. The levels of VEGF in the supernatant of HEC-1A after transfected with GFP plasmid, full- length and RNAi β-arrestin 2 plasmids were determined by ELISA method. Results The blVD and β-arrestin 2 expres-sion were higher in endometrial cancer specimens than that in normal endometrium and atypical endometrial hyperplasia specimens. Over-expression of β-arrestin 2 might cause higher level of VEGF secretion. Conclusions β-arrestin 2 is as- sociated with tumor angiogenesis of endometrial cancer but the mechanism needs to be further studied.

关 键 词：子宫肿瘤 Β-ARRESTIN 2 微血管密度 血管内皮生长因子 

分 类 号：R711.74[医药卫生—妇产科学]