砒霜对MRL/lpr自发性狼疮小鼠的生存时间及血清抗ds-DNA水平的影响  

Effects of Arsenic Trioxide on the Survival Time and Serum anti-ds-DNA Levels in MRL/lpr Lupus Mice

作　　者：王红[1] 谢奇朋[2] 

机构地区：[1]温州医学院附属第二医院肾内风湿免疫科,浙江温州325000 [2]温州医学院附属第二医院检验科,浙江温州325000

出　　处：《中华中医药学刊》2013年第2期308-310,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2010ZA090);温州市科技计划项目(Y20100158)

摘　　要：目的:探讨砒霜中的有效成分亚砷酸(Arsenic Trioxide,ATO)对MRL/lpr自发性狼疮小鼠的生存时间及血清抗ds-DNA水平的影响。方法:12周龄(发病期)MRL/lpr狼疮小鼠40只,设C57正常小鼠40只做参照,随机平分两组,即治疗(亚砷酸)组和对照(生理盐水)组,亚砷酸组ATO 0.4 mg/(kg.d)隔日腹腔注射,生理盐水组隔日腹腔注射同等量生理盐水,疗程至120 d结束,观察至210 d,比较亚砷酸组和生理盐水组在死亡率上的差异;采用ELISA法检测小鼠血清抗ds-DNA抗体的表达水平。结果:对两组MRL/lpr小鼠及C57小鼠的死亡率及血清抗ds-DNA抗体的水平的观察,结果发现MRL/lpr小鼠亚砷酸组累积死亡率(35%)低于生理盐水组(65%);血清抗ds-DNA抗体(OD值)亚砷酸组(0.9163±0.0667)低于生理盐水组(1.7975±0.2631,P<0.01)。生理盐水组为1.7975±0.2631,亚砷酸组为0.9163±0.0667,低于生理盐水组(P<0.01)。而C57小鼠中没有发现在生理盐水组及亚砷酸组中有统计学差别。结论:砒霜(ATO)可降低MRL/lpr小鼠的死亡率,延长其生存时间,同时可以使其外周血抗ds-DNA抗体的表达水平降低。Objective ：To investigate the effect of arsenic trioxide （ATO）on the survival time and serum anti -ds - DNA levels in MRL/lpr lupus mice. Methods ：40 MRL/lpr mice（ 12 weeks old）and 40 C57 mice were equally divided to 2 groups randomly：control group（saline）and treatment group [ arsenic trioxide 0.4 mg/（ kg. d）]. These drugs were given by abdominal injection for 120 days. Then these mice were observed until the 210th day. The indexes such as mortality,the serum levels of anti - double stranded DNA antibody were detected by enzyme - linked immunosorbent assay （ELISA）. Results：By comparing the mortality, anti -double stranded DNA antibody of mice in both groups,we found that the mortality of MRL/lpr mice in treatment group（ 35% ）was much lower than that in control group（65% ）, the mean OD value of serum anti - dsDNA antibody was lower in the ATO group （ 0. 9163 ± 0. 0667 ） than that in the control group （ 1. 7975 ＋0. 2631 ,P 〈0.01 ） ,all the indexes of C57 mice had no significant difference between two groups. Conclusion： An adequate dose of arsenic trioxide cart prolong the survival time of MRL/lpr lupus mice, reduce mortality, decrease the peripheral level of anti - dsDNA antibody expression.

关 键 词：砒霜 系统性红斑狼疮 生存时间 抗DS-DNA 

分 类 号：R285.5[医药卫生—中药学]