HPLC法测定蒙药清咽六味散中甘草酸的含量及TLC鉴别  

HPLC Method Measures the Content and TLC Identification of Glycyrrhizic Acid in Mongolian Medicine Qing Yan Liuwei San

作　　者：包桂花[1] 玉华[1] 包同力嘎[1] 

机构地区：[1]内蒙古民族大学,内蒙古通辽028300

出　　处：《中华中医药学刊》2013年第2期402-404,I0017,I0018,共5页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:建立蒙药清咽六味散中甘草酸的含量测定及薄层色谱鉴别方法。方法:采用高效液相色谱法,应用(Diamonsil C18 5μm,4.6 mm×250 mm);流动相:甲醇-0.2 mol/L醋酸铵溶液-冰醋酸(67∶33∶1);检测波长:250 nm;流速:1.0 mL/min;柱温:30℃;采用薄层色谱法对处方中的丁香、甘草、木香、诃子进行了鉴别。结果:甘草酸在0.506 2～3.543 8μg范围内与峰面积具有良好的线性关系,甘草酸的平均回收率为100.45%,RSD为0.82%(n=6);薄层色谱鉴别中各味药的特征斑点清晰,专属性强。结论:含量测定方法简便,精密度高,分离度好;薄层色谱鉴别方法专属性强,无干扰,可作为该制剂的质控方法。Objective： Establish the Content determination and thin -layer- chromatographic （TLC）identification methods of Glycyrrhizie acid in Mongolian medicine Qing yan liu wei Sail. Methods： Using high - performance liquid chromatography （ HPLC ）. Application （ Diamonsil C 18 5 μm 4.6 mm × 250 mm） ; Mobile phase ： Methanol - 0.2mol/L Ammonium acetate solution- Glacial acetic acid（67：33 ： 1 ） ;Detection wavelength ：250 nm;Veloeity：1. 0 mL/min; Column temperature：30 ℃ ; Using thin - layer - chromatography identifies Clove, iliquorice, eostus root, and Terminalia chebula in prescription. Results ： Glycyrrhizic acid and the peak area has a good linear relationship Within 0. 506 5 - 3. 543 8 μg. The average recovery rate of Glycyrrhizic acid is 100.45% , and RSD is 0.82% （ n = 6）. Thin - layer - chromatographic identification characteristic spots of the herbs is clear and strong exclusive. Conclusion： Content determi- nation method is simple, high precision, good separation. The identification method of Thin - layer - chromatographic is strong exclusive, without interference, could serve as the preparation quality control methods.

关 键 词：清咽六味散 甘草酸 HPLC法 TLC鉴别法 

分 类 号：R284.3[医药卫生—中药学]