慢性乙肝患者血清HBV cccDNA检测的临床意义  

Clinical significance of detecting serum HBV covalently closed circular DNA in patients with chronic hepatitis-B

作　　者：杨彩娥[1] 黄丽琴[2] 孙彬[1] 何菊芳[1] 韩冰[1] 

机构地区：[1]解放军第309医院检验科,北京100091 [2]河北北方学院,河北张家口075000

出　　处：《解放军医学院学报》2013年第2期121-123,共3页Academic Journal of Chinese PLA Medical School

摘　　要：目的探讨慢性乙肝患者血清HBV cccDNA与现有血清学指标(AST、ALT、TBIL、HBeAg、HBV DNA)的相关性。方法选取解放军第309医院2009年5月-2012年5月就诊的慢性乙肝患者58例,采用Roche C6000电化学发光法检测HBeAg;日立Hi7600全自动生化分析仪检测ALT、AST、TBIL;采用实时荧光定量PCR检测血清HBV DNA、HBVcccDNA载量。结果血清HBV cccDNA与HBV DNA有很好的相关性(r=0.880,P=0.000)。HBeAg阳性组HBV cccDNA阳性的比例为67.6%,显著高于HBeAg阴性组的37.5%(χ2=5.170,P=0.023)。血清HBV cccDNA与肝组织炎症的严重程度呈正相关(χ2=4.819,P=0.028)。结论血清HBV cccDNA定量检测是评价HBV复制情况和肝细胞损伤程度较为可靠的指标。Objective To study the correlation between serum HBV covalently closed circular DNA（ccc DNA） and current serum levels of AST,ALT,TBIL,HBeAg and HBV DNA in chronic hepatitis-B（CHB） patients.Methods Fifty-eight CHB patients admitted to Chinese PLA 309 Hospital from May 2009 to May 2012 were enrolled in this study.Their serum levels of HBeAg were measured with Roche C6000 electrochemiluminescence method,their serum levels of ALT,AST and TBIL were measured with Hitachi Hi7600 automatic biochemical analyzer,and their serum levels of HBV DNA and HBV cccDNA were measured by RT-PCR.Results The serum HBV cccDNA was closely correlated with the serum HBV DNA（r=0.880,P=0.000）.The proportion of positive HBV cccDNA was significantly higher in HBeAg-positive patients than in HBeAg-negative patients（67.6% vs 37.5%,χ2=5.170,P=0.023）.The serum HBV cccDNA was positively correlated with the severity of liver inflammation（χ2=4.819,P=0.028）.Conclusion Quantitative detection of serum HBV cccDNA is a reliable index for the assessment of HBV replication and liver cell damage.

关 键 词：慢性乙型肝炎 共价闭合环状DNA HBVDNA 乙肝E抗原 谷丙转氨酶 

分 类 号：R446.52[医药卫生—诊断学]