肌红蛋白基因多态性与运动性肌肉损伤的相关性  

Relationship between myoglobin gene polymorphism and exercise-induced muscle damage

作　　者：彭朋[1] 薄海[1] 张磊[1] 刁秋霞[1] 王大宁[1] 秦永生[1] 

机构地区：[1]武警后勤学院军事训练医学教研室,天津300162

出　　处：《武警医学》2013年第2期97-100,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：武警后勤学院博士启动基金(WYB201106);武警后勤科研项目(WJHQ2012-21)

摘　　要：目的探讨肌红蛋白(myoglobin,Mb)基因第2外显子单核苷酸多态(A79G)与运动性肌肉损伤的关系。方法85名武警战士进行一次大强度负重运动,检测血清肌酸激酶(creatine kinase,CK)活性的时程变化;聚合酶链反应-限制性片段长度多态(PCR-RFLP)法测定Mb基因多态性;分析基因多态性与安静CK(CKpre)、峰值CK(CKpeak)及CK最大变化值(△CK)的关系。结果 CKpre为(145±33)U/L,大强度运动后24 h开始逐渐升高,72 h达峰值[CKpeak:(2972±1648)]U/L,随后缓慢下降,120 h仍高于安静水平。3种基因型的分布频率分别为AA(54.1%)、AG(40.0%)、GG(5.9%),等位基因分布频率为A(74.1%)、G(25.9%),符合Hardy-Weinberg遗传平衡定律。组间比较,(AG+GG)组CKpre、CKpeak和△CK均高于AA组(均P<0.01)。结论运动性肌肉损伤易感性与Mb基因第2外显子单核苷酸多态(A79G)有关,G等位基因具有肌肉损伤易感性,而AA基因型则具有运动性肌肉损伤保护作用。Objective To study the relationship between single nucleotide polymorphism （A79G） and （T109C） in the second exon of the myoglobin gene and exercise - induced muscle damage. Methods Eighty - five soldiers of CAPF performed a strenuous weight -loading exercise and the time course of serum ereatine kinase （CK） activity was measured. Myoglobin gene polymorphism was detected by polymerase chain reaction -restriction fragment length polymorphism （PCR- RFLP） method. Association between resting CK （CKpre） , maximal CK （CKpeak）, maxiaml changes in CK （△CK） and gene polymorphism was then analysed. Results The ac- tivity of CK [ CKpre ： （145 ± 33） U/L]gradually increased at 24 h after strenuous exercise and reached peak value at 72 h [ CKpcak ： （2972 ± 1648） U/L], which then slowly reduced, but still higher than rest level at 120 h. The genotype frequencies （AA =54.1%, AG = 40.0%, GG = 5.9% ） and allele frequencies （ A = 74.1%, G = 25.9% ） were coincident with Hardy - Weinberg equilibrium. Compared between groups, CKpre, CKpeak and ACK in （AG ＋ GG） -group were all higher than those in AA genotype group （all P 〈 0.01 ）. Conclusions The single nucleotide polymorphism （A79G） in the second exon of the myoglobin gene might contribute to susceptivity of exercise - induced muscle damage. Subjects with G allele have increased risk for developing muscle damage, whereas AA genotype may have protective effects.

关 键 词：肌红蛋白 基因多态 运动性肌肉损伤 肌酸激酶 亚临床运动性横纹肌溶解症 

分 类 号：R821.5[医药卫生—临床医学]