慢性情绪应激对大鼠主动脉损伤的影响  

Effect of Chronic Emotional Stress on Aorta Injury in Rats

作　　者：赵咏梅[1] 王希柱[2] 刘宝义[2] 于秀艳[3] 陈秀清[1] 

机构地区：[1]开滦集团有限责任公司林西医院神经内科 [2]河北联合大学附属唐山人民医院 [3]开滦集团有限责任公司康复医院康复科

出　　处：《中国健康心理学杂志》2013年第1期152-154,共3页China Journal of Health Psychology

基　　金：唐山市科学技术研究与发展指导计划(项目编号:111302090a)

摘　　要：目的观察慢性情绪应激对大鼠主动脉的损伤作用。方法雄性Wistar大鼠40只,随机分为正常对照组(NC)、无应激组(NS)、生理应激组(PS)和情绪应激组(ES),每组各10只,并制作慢性情绪应激模型。于末次应激结束后采集血标本,全自动生化分析仪检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)和超敏C反应蛋白(hs-CRP),黄嘌呤氧化酶法检测血清超氧化物歧化酶(SOD)活力、硫代巴比妥酸法测定丙二醛含量、ELISA法测定血清Ox-LDL、放免法测定TNF-α水平;HE染色观察大鼠主动脉形态学变化。结果①血清学指标显示,ES组大鼠较其他3组出现了明显的脂代谢紊乱(TC、LDL-C、Ox-LDL水平明显升高,HDL-C下降)、氧化应激损伤(血清SOD活力明显下降而MDA含量明显升高)和炎症反应(血清hs-CRP和TNF-α升高);②HE染色显示ES组主动脉出现早期动脉粥样硬化性病理改变。结论慢性情绪应激可通过影响血脂、氧化应激和炎症反应,促进大鼠主动脉的损伤。Objective To investigate the effect of chronic emotional stress on aorta injury in rats.Methods A total of 40 male Wistar rats were divided into 4 groups（10 each）randomly,normal control group（NC）,no-stress group（NS）,physical stress group（PS） and emotional stress group（ES）.The chronic emotional stress model were made.The blood samples were immediately obtained after the last stress for assay of serum lipids,hs-CRP,SOD activity and MDA content.The level of TNF-α in serum was measured by radioimmunoassay kit and Ox-LDL was measured by the enzyme-linked immunosorbent assay.HE staining was used to detect the change of rat aorta.Results ① The ES group showed more significant dyslipidemia（increased TC,LDL-C,Ox-LDL;decreased HDL-C）,oxidative stress（decreased SOD activity and increased MDA）and inflammation（elevated hs-CRP and TNF-α,）than those in other groups.②HE staining showed the ES group had early atherosclerotic changes in aorta.Conclusion The results suggested Chronic emotional stress produce aorta injury by effecting serum lipid,oxidative stress and inflammation.

关 键 词：情绪应激 脂代谢紊乱 氧化应激 炎症 主动脉损伤 

分 类 号：R245.319[医药卫生—针灸推拿学]