荧光碳点共振光散射法测定核酸的应用研究  

Carbon dots used for the detection of nucleic acids with resonance light scattering signals

作　　者：董微[1] 王莹[2] 宋有涛[2] 徐淑坤[3] 

机构地区：[1]沈阳医学院化学教研室,辽宁沈阳110034 [2]辽宁大学生命科学院,辽宁沈阳110036 [3]东北大学化学系,辽宁沈阳110819

出　　处：《化学试剂》2013年第2期147-149,152,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(20875011);辽宁省自然基金资助项目(20102221);辽宁省教育厅资助项目(L2010542);辽宁省教育厅杰出青年学者成长计划资助项目(LJQ2011109);沈阳市科技局资助项目(F11-262-9-21)

摘　　要：以葡萄糖为碳源采用溶剂热法合成荧光碳点。利用共振光散射(RLS)技术研究了碳点与脱氧核糖核酸的相互作用,根据核酸在pH 8.8的Tris缓冲液中与碳点有很强的光散射信号,建立一种简便灵敏的测定核酸的方法。此方法中增强的RLS强度与核酸浓度在0.4～30μg/mL范围内呈线性关系,检出限为0.023μg/mL。将该法用于合成样品中核酸的测定,回收率在103%～106%之间,RSD<3.7%。Fluorescent carbon dots(CDs) were synthesized by a solvothermal method with glucose as the carbon source.The interaction between carbon dots and nucleic acids was studied with resonance light scattering technique.Based on the significant enhancement effect of the resonance light scattering between nucleic acids and carbon dots in the Tris buffer at pH 7.4,a convenient and feasible method for the determination of nucleic acids was proposed.The enhanced RLS intensity was found to be linearly to the concentration of nucleicacids in the ranges of 0.4 ～ 30 μg / mL,the detection limit of nucleic acids of 0.023 μg / mL(3σ).The method was applied to the detection of the nucleic acids in synthetical sample,with the recovery from 103% ～ 106% and the RSD lower than 3.7%.

关 键 词：碳点 共振光散射法 核酸 

分 类 号：O657.3[理学—分析化学]