两面针红外指纹图谱与抗癌活性的谱效研究  

Infrared fingerprint of Zathoxylum nitidum and its effect on inhibition of tumor cell

作　　者：毛晓丽[1,2] 覃禹[2] 蔡鹃[1,3] 郑娟梅 叶月华 刘华钢[5] 黄庶识[1] 

机构地区：[1]广西科学院生物物理实验室,广西南宁530007 [2]广西柳州市人民医院药剂科,广西柳州545006 [3]广西中医学院药学院,广西南宁530001 [4]广西区食品药品检验所中药室,广西南宁530021 [5]广西医科大学药学院,广西南宁530021

出　　处：《红外与毫米波学报》2013年第1期91-96,共6页Journal of Infrared and Millimeter Waves

基　　金：国家自然科学基金(30760302);广西科学研究与技术开发计划项目资助(0992003A-20,0992003A-21)~~

摘　　要：研究两面针的红外指纹图谱与抗肿瘤活性之间的相互关系.基于不同产地的两面针氯仿提取物的红外指纹图谱特征峰强度及其抗肿瘤活性的效果,采用后退法构建以两面针抑制人胃腺癌7901和人宫颈癌Hela两种肿瘤细胞株的谱效模型.所建立数学模型的预测值与实际测量值的偏差率全部在10%以内,说明两面针红外指纹图谱和两面针抗肿瘤活性之间具有相关性.结果表明,生物碱类成分在两面针抗肿瘤药效活性中发挥了重要的作用.Mathematical expression was established for evaluating anticancer efficacy of samples of Z. nitidum to human tumor cells based on their infrared fingerprints. Chloroform extracts of the root of Z. nitidum from different habitats in China were used as subject samples to do anticancer experiments on two strains of human tumor cells, 7901 and Hela cells. Meanwhile, their fingerprints of infrared spectra were obtained. Screening arguments of backward elimination method was adopted to establish the spectra-effect correlation between infrared fingerprint and pharmacology data. The results showed that deviations of the predictive values from the true values were within ± 10% for the two kinds of tumor cells, suggested that there was close correlation between infrared fingerprint and biological activity of Z. nitidum. The model implied that alkaloids in Z. nitidum have main effect on tumor cells.

关 键 词：光谱分析 两面针 红外指纹图谱 谱效关系 

分 类 号：O657.33[理学—分析化学] R285.5[理学—化学]