冰草属植物种质资源遗传多样性的ISSR分析  

Genetic diversity analysis of Agropyron germplasm resources by ISSR

作　　者：曾亮[1,2] 袁庆华[1] 王方[1,3] 王瑜[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]甘肃农业大学草业学院,甘肃兰州730070 [3]兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2013年第1期260-267,共8页Acta Prataculturae Sinica

基　　金：国家科技支撑项目(2011BAD17B01)资助

摘　　要：用ISSR标记对来自国内外的33份冰草材料的遗传多样性进行了检测。从93条ISSR引物中共筛出11条能扩增出清晰条带并具有多态性的引物,33个样品DNA共获得84个扩增位点,其中多态性位点59个,平均每个引物扩增位点为7.64个。品种间遗传相似系数在0.083～0.706,表现出丰富的遗传多样性。利用UPGMA聚类分析,以遗传相似系数0.52为界限,33份材料划分为4类,聚类基本符合地理来源相近的材料聚为一类,呈现出一定的地域性分布规律。The genetic diversity of 33 Agropyron materials from both at home and abroad was analyzed by ISSR（inter-simple sequence repeats） markers.Eleven primers were selected from 93 primers which had clear polymorphic bands.A total of 84 bands of which 59 were polymorphic were obtained from the 33 materials.On average,each primer amplification site was 7.64.The genetic similarity coefficient of varieties was between 0.083 and 0.706,and showed rich genetic diversity.UPGMA cluster analysis gave a genetic similarity coefficient of 0.52 for boundaries and the 33 materials were divided into four types,with basic clustering in accord with geographical origin and regional distribution.

关 键 词：冰草 ISSR标记 种质资源 遗传多样性 

分 类 号：S543.9[农业科学—作物学]