微波辅助提取黄皮叶黄酮类化合物及其种类初步鉴别  

Microwave-assisted extraction and primary identification of flavonoids from Clausena lansium leaves

作　　者：张福平[1] 刘晓珍[1] 汤艳姬[1] 陈焕彬 吴远清 

机构地区：[1]韩山师范学院生物系,广东潮州521041 [2]潮州市新星农业开发有限公司,广东潮州515632

出　　处：《南方农业学报》2013年第1期126-130,共5页Journal of Southern Agriculture

基　　金：广东省教育部产学研结合项目(2011B090400121)

摘　　要：【目的】研究黄皮叶黄酮类化合物的提取工艺条件及鉴别其黄酮种类,为黄皮叶的开发利用提供依据。【方法】采用微波辅助提取黄皮叶黄酮类化合物,通过单因素试验及正交试验优化乙醇浓度、浸提温度、微波功率和微波时间等提取参数,同时还对黄皮叶黄酮类化合物进行初步鉴别。【结果】对黄皮叶黄酮类化合物提取率影响较大的因素是乙醇浓度和浸提温度,其最佳提取工艺为:乙醇浓度75%、固液比1∶20、浸提温度55℃、浸提时间1.5h、微波功率160W、微波时间40s,该提取工艺条件下,黄皮叶总黄酮类化合物提取率达3.28%,比常规方法高0.27%(绝对值)。通过颜色反应鉴别,初步确定黄皮叶提取液中黄酮的种类主要为双氢黄酮、查尔酮和黄酮醇等。【结论】以微波辅助提取黄皮叶黄酮类化合物具有操作简单、成本低、用时短、稳定性好等特点,值得开发利用。【Objective】This research studied the extraction conditions and primary identification of flavonoids from the Clausena lansium leaves in order to provide basis for the development and utilization of Clausena lansium leaves. 【Method】 Using the microwave-assisted extraction method， the flavonoids from Clausena lansium leaves were extracted， and relevant parameters， including ethanol concentration， extraction temperature， microwave power， and extraction time， were tested through the single factor experiment and orthogonal test. At the same time， flavonoids from Clausena lansium leaves were preliminarily identified. 【Result】The extraction rate of flavonoids from Clausena lansium leaves was mainly affected by the ethanol concentration and extraction temperature， and the optimum extraction condition was 75% ethanol concentration， 1∶20 solid-to-liquid ratio， 55℃ extraction temperature， 1.5 h extraction time， 160 W microwave powers， and 40 s microwave time. Under the given optimal extraction condition， the total flavonoids extraction rate reached 3.28%， which was 0.27% （absolute value） higher than traditional extraction methods. Through the color reaction identification， the liquid extractions of flavonoids in Clausena lansium leaves were mainly identified as flavanone， chalcone， flavonol， etc. 【Conclusion】Extraction of flavonoids from Clausena lansium leaves by the microwave-assisted method was easy to operate， money-saving， time-saving， and stable， so the method was worthy of development and utilization.

关 键 词：黄皮叶 黄酮类化合物 微波辅助提取 鉴别 

分 类 号：R284.2[医药卫生—中药学]