脾肾两虚型脑瘫差异表达基因筛选与初步验证  

Screening and Preliminary Verification to Differentially Expressed Genes from Cerebral Palsy with Spleen and Kidney Deficiency Syndrome

作　　者：杨红亚[1] 张天娥[1] 鲁法庭[1] 刘伟伟[1] 陈晓玲[1] 董小丽[2] 王米渠[1] 

机构地区：[1]成都中医药大学,四川成都610075 [2]成都市第一人民医院,四川成都610016

出　　处：《云南中医学院学报》2013年第1期8-11,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(NO:30672579;NO:81072727);四川省教育厅项目(NO:09ZA031;NO:08ZA120)

摘　　要：目的筛选并验证与脾肾两虚型脑瘫相关的差异表达基因。方法从前期正常-脾肾两虚型脑瘫双生子基因芯片中获得的差异表达基因中筛选15个病证相关特征基因,选取11例脾肾两虚型脑瘫患儿与10例健康者分别为实验组和对照组,采用RT-PCR对筛选到的特征基因的表达情况进行研究。结果 FIGN和PTGER2在实验组和对照组的表达水平存在明显差异。其中,FIGN在脾肾两虚型脑瘫患儿中的表达低于对照组(P<0.01),PTGER2的表达高于对照组(P<0.01)。结论 FIGN和PTGER2的异常表达与脾肾两虚型脑瘫的发生有相关性,提示其可能为该病证的候选标志分子。Objective To screen and verify differentially expressed genes from Cerebral Palsy with spleen and kidney defi- ciency syndrome. Methods 15 characteristic genes related with the disease and syndrome were chosen to be verified from the differ- entially expressed genes screened from earlier cDNA microarray of ＂normal-CP＂ twin with spleen and kidney deficiency syndrome. 11 Cerebral Palsy with spleen and kidney deficiency syndrome children were selected as experimental group, and 10 healthy children were selected as control group. The expression of screened differential genes was compared between two groups through RT-PCR test. Results The expressed level of FIGN and PTGER2 had the significant differences between experimental group and control group. FIGN was significantly low expressed, and PTGER2 was significantly high expressed in experimental group compared to that of the control group （P〈0.01）. Conclusion There is correlation between the abnormal expression of FIGN and PTGER2 and Cerebral Palsy with spleen and kidney deficiency syndrome .It can be inferred that the two genes may be the candidate marker-gene of Cerebral Palsy with spleen and kidney deficiency syndrome.

关 键 词：脾肾两虚 脑瘫 差异表达基因 RT-PCR 

分 类 号：R272[医药卫生—中医儿科学]