茜素黄R-γ-环糊精包合物与鲱鱼精DNA的作用机制  

Investigation of Interaction Between γ-Cyclodextrin-Alizarin Yellow R Clathrate Compound and DNA

作　　者：欧亮龙[1] 王兴明[1] 

机构地区：[1]西南科技大学材料科学与工程学院化学系,四川绵阳621010

出　　处：《中山大学学报（自然科学版）》2013年第1期89-93,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30973634)

摘　　要：在生理pH=7.4环境下,用摩尔比法测定γ-环糊精(γ-CD)与茜素黄R(AYR)的包合比(摩尔比)nγ-CD∶nAYR=1∶1,确定包合常数Kοf,288.15K=1.69×105L/mol。以吖啶橙(AO)作为分子探针,用紫外光谱法、荧光光谱法、化学热力学法和黏度法等研究包合物γ-CD-AYR与鲱鱼精DNA(hsDNA)的作用,得到γ-CD-AYR包合物与hsDNA作用的结合比nDNA∶nγ-CD-AYR=1∶2,结合常数为K288.15K=3.10×104L/mol,K310.15K=4.02×104 L/mol。热力学函数ΔrHmο=8.78×103J/mol,ΔrGmο=-2.59×104 J/mol,ΔrSmο=116.45 J/(mol.K),说明γ-CD-AYR包合物与DNA作用为熵驱动。确定γ-CD-AYR与hsDNA的作用方式为静电和部分嵌插的作用方式。Under the condition of physiological environment at pH 7.4, the binding ratio n.t.CD： nAyR =1：1 and binding constant K ,28s 15K 1.69 105 L/mol were confirmed by mole ratio method and linear method. AO was used as molecule probe, and the interaction between -CD-AYR and herring-sperm DNA was studied by UV spectra, fluorescence spectra, thermodynamic method and viscosity method, etc. A series of thermodynamic data such as ArHm = 8.78 X 103J/mol, ArGm = -2. 59 x 104 J/tool, ArSme = 116.45 J/ （mol K） were obtained, and ArSm was the primary driven power of this interac- tion, the binding ratio was nDNA： n_CD_AyR = 1：2, the binding constants were K288.15K = 3.10 104 L/mol, g310.15K = 4. 02 104 L/mol. The interaction mode between ＂-CD-AYR and herring-sperm DNA was an electrostatic interaction and part embedded intercalation.

关 键 词：茜素黄R Γ-环糊精 包合物 鲱鱼精DNA 作用方式 

分 类 号：O629.74[理学—有机化学]