高效液相色谱法测定清开灵胶囊中绿原酸和黄芩苷的含量  

Quantitative Determinnation of Chlorogenic Acid and Baicalin inQingkailing Granule by HPLC

作　　者：乔岩岩[1] 彭家钢[1] 朱江 杨瑞芬[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北省随州市食品药品监督检验所,湖北随州441300

出　　处：《湖北中医药大学学报》2013年第1期25-27,共3页Journal of Hubei University of Chinese Medicine

摘　　要：目的建立高效液相色谱(HPLC)法同时测定清开灵胶囊中绿原酸和黄芩苷含量分析方法。方法用C18硅胶柱(150mm×4.6mm,5μm)为固定相,甲醇-0.4%的磷酸水溶液为流动相,进行梯度洗脱,流速为1mL/min,紫外检测波长为323nm。结果绿原酸和黄芩苷的线性范围分别为4.248-106.2μg/mL,9.224-203.6μg/mL;相关系数分别为0.999 7和0.999 8;加样回收率分别为101.23%和100.72%;检出限分别为0.048μg,0.034μg;精密度实验RSD分别为0.75%,1.88%;重现性实验RSD分别为1.99%,2.73%;稳定性实验RSD分别为1.8%,0.52%。4批样品中绿原酸的含量为0.2215%-0.2594%,黄芩苷的含量为4.0947%-4.3743%。结论该方法简便、快速、准确,灵敏度高,重现性好,成本低,可用于清开灵胶囊的质量控制。Objective To develop an RP-HPLC method for the determination of chlorogenic acid and baicalin in Qingkailing Granule.Methods A C18（150mm×4.6mm,5μm） bond silica for reersed-phase HPLC was prepared.The mobile phase was CH3OH-0.4% Phosphoric acid.The flow rate was set at 1.0mL/min.The detection wavelength was at 323 nm.Chlorogenic acid and baicalin were separated by HPLC with grade elution.Results The linearity ranges of chlorogenic acid and baicalin were 4.248-106.2μg/mL and 9.224-203.6μg/mL respectively;The correlation coefficient were 0.9997 and 0.9998;The recoveries of adding sample rate were 101.23% and 100.72%;The detection limits were 0.048μg and 0.034μg.The RSD（n=5） of measurement precisions test were 0.75% and 1.88%.The RSD（n=5） of reproducibility between tests were1.99% and 4.73%.The RSD（n=5） of stability test were 1.8% and 0.52% The content of chlorogenic acid in compound Qingkailing granule was 0.2215%-0.2594%,and that of baicalin was 4.0947%-4.3743%.Conclusion The method is simple,fast,accurate,sensitive,reproducible and low cost.It is fit for the quality control of compound Qingkailing Granule.

关 键 词：清开灵胶囊 绿原酸 黄芩苷 高效液相色谱法 梯度洗脱 

分 类 号：R284[医药卫生—中药学]