细胞外基质磷酸糖蛋白对细胞增殖能力的影响  

Study on the role of MEPE in cell multiplication and migration

作　　者：刘爽[1] 刘占领[1] 张淑红[1] 张辉[1] 侯杰[1] 罗兰[1] 魏巍[1] 

机构地区：[1]佳木斯大学,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2013年第1期1-2,共2页Heilongjiang Medicine and Pharmacy

基　　金：中国教育部归国留学人员启动基金(052号文件);黑龙江省自然科学基金项目(D200946);黑龙江省卫生厅面上项目(2009-354);黑龙江省教育厅项目(1251G059)

摘　　要：目的:探讨细胞外基质磷酸糖蛋白对细胞增殖能力的影响。方法:构建含细胞外基质磷酸糖蛋白基因的质粒,稳定转染293T细胞,通过激光共聚焦显微镜与RT-PCR检测转染细胞株hMEPE蛋白表达结果。通过MTT实验检测细胞增殖与迁移能力。通过RT-PCR检测p53基因的表达水平的差异,进一步探讨肿瘤细胞增殖的分子机制。结果:与转染空载体细胞相比,稳定转染hmepe细胞株增殖能力有明显提高,RT-PCR结果显示p53基因在转染目的基因细胞中较转染空载体细胞其表达增高。结论:细胞外基质磷酸糖蛋白可以提高细胞增殖能力。Objective：To observe the expression of MEPE during cell culture, and investigate MEPE func- tion. Methods：An experimental model of fracture was established, and MEPE were detected by RT--PCR in different stage of intracebranous. 293T was transfected by pLEGFP--C1--MEPE and then MEPE was detect- ed by immune. Results：The results demonstrated that in the stage of early bone formation, the level of MEPE mRNA was higher, and MEPE can enhance cell multiplication and migration. Conclusion： MEPE is upstage regular pathway of cell multiplication and migration.

关 键 词：细胞外基质磷酸糖蛋白 增殖 

分 类 号：Q25[生物学—细胞生物学] Q786