来氟米特对阿霉素肾病大鼠尿、肾脏VEGF表达的影响  

The influence of leflunomide on the expression of urine and kidney VEGF of adriamycin nephropathy rats

作　　者：邹艳红[1] 迟瑛娇[1] 赵塔娜[1] 聂磊[1] 王禹[1] 王李鸣[1] 赵冬梅[1] 李春玉[1] 

机构地区：[1]佳木斯大学附属第一医院儿科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2013年第1期48-49,共2页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省卫生厅科研课题;编号:2011-393

摘　　要：目的:探讨来氟米特对阿霉素肾病大鼠尿、肾脏血管内皮生长因子(VEGF)表达的影响。方法:将56只清洁级雄性Wistar大鼠随机分为正常对照组(生理盐水组)、肾病组(阿霉素组)、治疗组(来氟米特组)。建立阿霉素肾病大鼠模型,各组分别于第2周、4周、6周处死大鼠,取尿、肾脏,用ELISA方法检测并做相关分析。结果:肾病组在第2周、4周、6周时VEGF在尿、肾组织中的含量均显著高于正常对照组(P<0.01);且均与24h尿蛋白量呈正相关(r=0.81;0.63;P<0.01);来氟米特(LEF)干预组上述指标均显著低于肾病组,差异有统计学意义(P<0.01)。结论:来氟米特对阿霉素肾病大鼠肾脏损害有保护作用,其机制可能是通过降低VEGF表达而实现。Objective：To examine the effects of leflunomide on urine, renal vascular endothelial growth fac- tor （VEGF） expression of adriamycin nephropathy rats. Methods： 56 clean male Wistar rats were randomly di- vided into normal control group （saline group）, nephropathy （ADR group）, and treatment group （LEF group）. To establish adriamycin rat models, the rats in each group were killed in the first two weeks, four weeks, six weeks; the urine and kidney were taken and analyzed by the ELISA method. Resul ts：For nephropa- thy group at second weeks, 4th weeks, 6th weeks, VEGF in urine and renal tissue were significantly higher than those in normal control group （P〈0.01）; and all the amount of urinary protein was positively correlated with 24h （r=0. 81 ; 0. 63 ;P 〈0.01） ; Leflunomide（LEF） intervention group were significantly lower than the index of nephrosis group; the difference was statistically significant （P〈0.01）. Conclusion：Leflunomide has a protective effect on rats with adriamycin kidney damage, and its mechanism may be achieved by reducing the expression of VEGF.

关 键 词：来氟米特 阿霉素肾病 VEGF 

分 类 号：R965[医药卫生—药理学] R692[医药卫生—药学]