松针叶绿素-胡萝卜素软膏体外抗脂质过氧化作用的研究  

Anti-lipid Peroxidation of Chlorophyll-carotene Paste from Pine Needles in Vitro

作　　者：郑光耀[1,2,3] 何玲[4] 薄采颖[1,2,3] 谢衡 刘冬芹[4] 李若达 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [4]中国药科大学药理学教研室,江苏南京210009 [5]中健行集团有限公司,广东乐昌512229

出　　处：《林产化学与工业》2013年第1期45-48,共4页Chemistry and Industry of Forest Products

基　　金：科技部农业科技成果转化资金项目(2008GB24320412);教育部'新世纪优秀人才'支持计划资助项目(NCET-10-0817);国家'十二五'重大新药创制专项(2012ZX09504001-001)

摘　　要：探讨松针叶绿素-胡萝卜素软膏的体外抗脂质过氧化作用,在大鼠肝匀浆和肝线粒体中加入不同浓度的松针叶绿素-胡萝卜素软膏,观察它们对大鼠肝匀浆自发性过氧化、VC-Fe2+诱导肝匀浆和肝线粒体脂质过氧化的丙二醛(MDA)抑制效果。结果表明:松针叶绿素-胡萝卜素软膏能有效抑制大鼠肝匀浆的自发性和诱导性脂质过氧化产生的MDA,其抑制率为50%时药物浓度(IC50)分别为398.72和355.05 mg/L,对肝线粒体的诱导性脂质过氧化产生的MDA也有较好的抑制作用,其IC50为687.02 mg/L,还能减轻肝线粒体的肿胀程度,且呈现良好的量效关系。Anti-lipid peroxidation of chlorophyll-carotene paste from pine needles was investigated in vitro. MDA production in rat liver homogenate and mitochondria spontaneously formed or induced by Vc-Fe^2＋ system were measured. The results showed that the chlorophyll-carotene paste could inhibit the generation of MDA in liver homogenate under spontaneous and induced conditions, as well as in liver mitochondria under induced condition, with IC50 of 398.72 mg/L, 355.05 mg/L and 687.02 mg/L, respectively. The swelling of liver mitochondria was also inhibited with dose-response relationship. It is suggested that chlorophyll-carotene paste from pine needles possessed anti-oxidant activity in vitro.

关 键 词：松针 提取物 肝匀浆 线粒体 脂质过氧化 

分 类 号：TQ35[化学工程] R961[医药卫生—药理学]