HPLC法同时测定凤尾草中5种黄酮类成分的含量  

Simultaneous Determination of Five Flavonoids in Pteris multifida Poir.by HPLC

作　　者：刘建群[1] 任晓静[1] 舒积成[1] 张锐[1] 张维[1] 潘景行[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《林产化学与工业》2013年第1期117-120,共4页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(30960495)

摘　　要：建立高效液相色谱法,同时测定10个不同产地凤尾草中5种黄酮类成分:新西兰牡荆苷、忍冬苷、野漆树苷、木犀草素和芹菜素的含量。使用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱(0～10 min,15∶85;20～30 min,25∶75;35～40 min,50∶50),流速1.0 mL/min,检测波长350 nm,柱温25℃。结果新西兰牡荆苷、忍冬苷、野漆树苷、木犀草素和芹菜素5个黄酮类化合物在测定的范围内峰面积与浓度之间均表现出良好的线性(r≥0.999,n=5),加样回收率分别为97.18%～105.20%(RSD为1.72%～3.20%)、95.02%～104.92%(RSD为1.96%～2.93%)、98.45%～103.70%(RSD为1.66%～2.70%)、96.41%～104.73%(RSD为1.03%～3.46%)、95.82%～99.34%(RSD为1.85%～3.95%)。结果表明凤尾草中5种黄酮含量的地区差异性不大,各地区凤尾草中5种黄酮类化合物总含量在0.5～2.2 mg/g范围内。An HPLC method was established to the determination of five flavonoids （ vicenin-2, lonicerin, rhoifolin, luteolin and apigenin） in Pteris multifida. Separation was carried out on a Diamonsil C18 column （4.6 mm × 250 mm, 5 μm）. The mobile phase was acetonitrile-water （ containing 0.1% formic acid） with gradient elution （0 - 10 min, 15 ： 85 ; 20 - 30 min, 25 ： 75 ; 35 - 40 min, 50 ： 50）. The flow rate was 1.0 mL/min at 25 ℃, and the detection wavelength was set at 350 nm. The average recoveries for vicenin-2, lonicerin, rhoifolin, luteolin and apigenin were 97. 18% - 105.20% （ RSD1. 72% - 3. 20% ）, 95.02% - 104.92% （ RSD 1.96% - 2.93% ）, 98.45% - 103.70% （ RSD 1.66% - 2.70% ）, 96.41% - 104.73% （ RSD 1.03 % - 3.46% ）, 95.82% -99.34% （ RSD 1.85 % - 3.95% ）, respectively. These compounds showed good linearity （ r ≥ 0. 999, n = 5） in the detected concentration ranges. There were no significant differences of mass factions of the five flavonoids from different regions. The total mass factions of the five flavonoids were in the range of 0. 5 - 2.2 mg/g.

关 键 词：凤尾草 黄酮 高效液相色谱法 

分 类 号：TQ35[化学工程] R285.5[医药卫生—中药学]