绵羊肺腺瘤kras和其他相关基因突变的检测  

Mutation Detection of kras and Other Related Genes in Ovine Pulmonary Adenomatosis

作　　者：周艳喜[1] 于立新[1] 张月梅[1] 敖威华 朱富余[1] 么宏强[1] 马学恩[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古出入境检验检疫局,内蒙古呼和浩特010020

出　　处：《动物医学进展》2013年第2期6-11,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31060332)

摘　　要：为了解绵羊肺腺瘤与基因突变的关系,从绵羊肺腺瘤病毒感染的病羊肺脏组织扩增kras、nras、pik3ca、egfr、p53基因突变频率高的位点所在外显子核苷酸序列,应用PCR-SSCP技术和测序分析检测基因突变。结果显示,nras、pik3ca、egfr基因未检测到突变。2个阳性样品kras基因第1外显子检测出突变,核苷酸突变为点为35G→T,编码的氨基酸位于第12密码子为12G→V。1个阳性样品p53基因第7外显子检测出突变,核苷酸突变位点为825T→C,编码的氨基酸未发生突变,依然是C。为绵羊肺腺瘤病毒(OPAV)分子致病机制的研究提供了参考资料。To investigate the relationship between OPA（ovine pulmonary adenomatoms） and gene mutanon, the exons of genes nras,kras,pik3ca,egfr and p53 were amplicated by PCR. Gene mutation site was detected by using technique of PCR-SSCP（polymerase chain reaction-single strand conformation polymorphism） and sequencing. Mutation site was not found in genes nras,pik3ca and egfr. The mutation site was deteced in exonl of kras, there was a site mutation 35 G --T at 12 coden, and the cording amino acids 12 G-V. The mutation site was also found in exon7 of p53, 825 T--C,the cording amino acids still was 275 C- C. The results will be useful in further study of OPA molecular pathogenesis mechanism.

关 键 词：绵羊肺腺瘤病毒 RAS基因 P53基因 信号转导 

分 类 号：S852[农业科学—基础兽医学] Q789[农业科学—兽医学]