三个类群藏系绵羊线粒体细胞色素b基因序列多态性及其系统进化的研究  

Genetic Diversity and Evolution Relationship of Tibetan Sheep Inferred from mtDNA Cytb

作　　者：江容娥[1] 沈阅[2] 徐亚欧[1] 王永[2] 匡学谦[1] 钟金城[2] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]西南民族大学青藏高原生态保护与畜牧业高科技研发基地管委会,四川成都610041

出　　处：《动物医学进展》2013年第2期15-20,共6页Progress In Veterinary Medicine

基　　金：中央高校基本科研业务费专项(11NZYQN08)

摘　　要：为研究藏系绵羊红原、贾洛、欧拉3个类群的亲缘关系、遗传多样性以及其系统进化,测定该3个类群藏系绵羊的41个个体的细胞色素b基因的全序列。结果表明,41个藏系绵羊的细胞色素b基因的全序列长度均为1 140bp,有27个多态位点,其中单一多态位点16个,简约信息位点11个,共定义13种单倍型,单倍型多样性为0.672±0.078,核苷酸多样性为0.331%。藏系绵羊贾洛类群与欧拉类群遗传距离最远,为0.003 9,红原类群与欧拉类群遗传距离较近,为0.002 64。说明红原类群与欧拉类群亲缘关系较近,藏系绵羊有一个共同祖先,同时存在第2个母系起源。In order to investigate genetic diversity and phylogenetic relationships of Tibetan sheep,the complete cytb sequences of 41 individuals from 3 groups of sheep were sequenced. The results showed that the size of cytochrome b gene sequences of sheep are 1 140 bp. These sequences have 27 polymorphic loci, 16 are singleton variable sites, 11 are parsimony informative sites. Among identified 13 haplotypes, the haplotype diversity （Hd） and nucleotide diversity（Pi） were 0. 672± 0. 078 and 0. 331%. The genetic distance from Jialuo and Ola was 0. 0039 which are longer than that from Hongyuan and Ola. The plylogenetic relationships suggested that Tibetan sheep of Hongyuan subbreed are closely related with Ola. Tibetan sheep have two origins.

关 键 词：藏系绵羊 细胞色素B基因 起源 

分 类 号：Q789[生物学—分子生物学]