梅花鹿α干扰素全基因的克隆与分子进化分析  

Cloning and Phylogenetic Analysis of Interferon-α Gene of Sika Deer

作　　者：苏凤艳[1] 宗颖[1] 魏吉祥[1] 曾范利[1] 王全凯[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《动物医学进展》2013年第2期25-29,共5页Progress In Veterinary Medicine

基　　金：吉林省自然科学基金项目(201115194);吉林省教育厅项目[2009第(66)号]

摘　　要：根据GenBank中牛干扰素-α(IFN-α)基因序列(A00145),设计并合成了一对引物,以梅花鹿肝脏组织DNA为模板,采用PCR技术扩增梅花鹿IFN-α全基因,并克隆、测序。结果表明,梅花鹿IFN-α基因全长570bp,编码189个氨基酸,含有6个与二硫键形成有关的半胱氨酸;序列比较分析发现,梅花鹿IFN-α基因序列与GenBank发表的17种IFN-α基因核苷酸序列同源性为29.6%～93.7%,氨基酸序列同源性为18.4%～91.1%;梅花鹿与其他17种动物的IFN-α基因序列分析和系统进化树分析表明,IFN-α基因存在种属特异性,亲缘关系越近,同源性越高。梅花鹿INF-α基因的成功克隆,为进一步研究梅花鹿INF-α基因表达、生物学活性和应用奠定了基础。One pair of primers were designed and synthesized according to the bovine interferon alpha（IFN- a）gene nucleic acid sequence（A00145）published in the GenBank. The sika deer gene was amplified by PCR from genome DNA extracted from liver of sika deer, then cloned and sequenced. The result showed the full length sequence of sika deer IFN-a gene was consisted of 570 bp, which includes open-reading frame, encoding 189 amino acid residues,six cysteines in deduced amino acid sequence. Compared with 17 nucleotide sequences of IFN-a gene published in the GenBank, the homology is 29. 6%-93. 7%. Deduced amino acids homology is 18.4%-91.1%. The results of comparative sequence analysis and phylogenetic tree anal- ysis showed there was species difference in IFN-a gene, the nearer relationship,＇ the higher homology. This study set a basis for future studv of biological function and application of sika deer IFN-a.

关 键 词：梅花鹿 α干扰素基因 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]