枯草芽孢杆菌的分离筛选  

Isolation and Screening of Bacillus Subtilis

作　　者：白延琴 辛小玲 来有志 张效川 张刚娟 刘建峰 辛亚平[7] 

机构地区：[1]子长县畜牧兽医局史家畔畜牧兽医站,陕西子长717300 [2]富平县畜牧兽医工作站 [3]陕西正大有限公司 [4]吴起县畜牧局 [5]商南县畜牧中心赵川兽医站 [6]陕西洛南县四皓镇兽医站 [7]西北农林科技大学动物科技学院

出　　处：《畜牧兽医杂志》2013年第2期24-27,31,共5页Journal of Animal Science and Veterinary Medicine

基　　金：国家863项目(2010AA10Z101;2011AA100307;2011ZX08007-002);MATS-Beef Cattle Sys-tem以及陕西省农业攻关项目(2010K02-13)资助

摘　　要：本文基于从秸秆垛底层土壤中分离枯草芽孢杆菌的目的,为发酵秸秆提供菌株,从陕西杨凌农村秸秆垛中采取底层土样并进行分离。分离出11株菌,经过形态特征、生理生化特征等方法从中分离初步鉴定了3株疑似菌株。经对液态发酵条件的优化,对3株疑似菌进行液态发酵试验,以粗蛋白和粗纤维含量为标准,筛选出一株发酵效果最好的菌株b-1进行了16SrDNA序列分析,最终鉴定b-1菌株为枯草芽孢杆菌(Bacillus subtilis),其同源性≥99%。This study aimed to isolate Bacillus subtilis from the bottom soil of wheat straw and provide strains for straw fer- mentation, the soil sample was from Yangling Shaanxi. 11 strains were isolated,3 suspected strains were identified using morphological, physiological and biochemical characteristics methods. After optimizing of liquid fermentation, a best strain b--1 was screened with CP and CF as standard and 16S rDNA sequence was carried out,strain b--1 is considered to be Bacillus subtilis, and shows 99%homology.

关 键 词：枯草芽孢杆菌 筛选 鉴定 

分 类 号：S816.6[农业科学—饲料科学]