普通小麦–大赖草易位系T7BS·7Lr#1S和T2AS·2AL-7Lr#1S的分子细胞遗传学鉴定  

Molecular and Cytogenetic Identification of Triticum aestivum–Leymus racemosus Translocation Lines T7BS·7Lr#1S and T2AS·2AL-7Lr#1S

作　　者：崔承齐[1] 王林生[1,2] 陈佩度[1] 

机构地区：[1]南京农业大学/作物遗传改良与种质创新国家重点实验室/细胞遗传研究所,江苏南京210095 [2]河南科技大学农学院,河南洛阳471003

出　　处：《作物学报》2013年第2期191-197,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30871519);国家重点基础研究发展计划(973计划)项目(2009CB118304);江苏省优势学科建设项目资助

摘　　要：大赖草7Lr#1S染色体上携带赤霉病抗性基因,将其导入普通小麦有助于增加赤霉病抗源多样性和选育抗赤霉病品种。利用染色体C-分带、荧光原位杂交和分子标记技术从普通小麦–大赖草7Lr#1单体异附加系的花粉辐射后代中,选育出易位系T7BS·7Lr#1S(NAU639)和T2AS·2AL-7Lr#1S(NAU640)。经连续3年大田、温室赤霉病接种鉴定,这2个易位系的赤霉病抗性均显著高于感病亲本中国春、感病对照绵阳8545和石麦4185,为赤霉病抗病育种提供了新的种质资源。A resistance gene against wheat scab is located on chromosome 7Lr#1S of Leymus racemosus, which is propitious to enriching scab resistance resources and resistant breeding in wheat （Triticum aestivum L.）.The translocation lines NAU639 （T7BS·7Lr#1S） and NAU640 （T2AS·2AL-7Lr#1S） were selected from the plant progenies of irradiated pollens of monosomic alien addition line MA7Lr#1 and confirmed through chromosome FISH-GISH, C-banding, and molecular markers analyses. The two translocation lines had higher resistance to wheat scab than the susceptible parent “Chinese Spring” and the susceptible controls “Mianyang 8545” and “Shimai 4185” in a successive three-year test both in the greenhouse and field. These results showed the stable scab resistance in translocation lines NAU639 and NAU640 and feasibility to be used as a new source in wheat breeding resistant to scab disease.

关 键 词：普通小麦 大赖草 易位系 分子细胞遗传学 赤霉病抗性 

分 类 号：S512.1[农业科学—作物学]