溴氰菊酯在急性中毒大鼠体内的分布  

Distribution of Deltamethrin in Acute Poisoned Rats

作　　者：武斌[1] 严鹏[1] 尉志文[1] 王玉瑾[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001

出　　处：《法医学杂志》2013年第1期25-27,30,共4页Journal of Forensic Medicine

基　　金：山西省普通高等学校大学生创新性实验项目(2009-2010);山西省科技攻关项目(20110321082);"十一五"国家科技支撑计划项目(2007BAK26B05)

摘　　要：目的建立溴氰菊酯灌胃急性中毒大鼠模型及生物样品中溴氰菊酯的气相色谱-电子捕获检测器法（gaschromatography—electroncapturedetector，GC—ECD），研究溴氰菊酯在急性中毒大鼠体内的分布特征，为溴氰菊酯中毒案件的法医学鉴定提供参考依据。方法不同剂量（512和1024mg／kg）溴氰菊酯灌胃染毒大鼠后1．5h处死，迅速解剖取血液、心、肝、肺、肾、脑等，样品经无水硫酸钠研磨，混合溶剂[y（石油醚）：y（丙酮）=4：1]浸提，GC—ECD法定量检测溴氰菊酯含量。结果溴氰菊酯与内源性杂质分离良好，血液和肝中溴氰菊酯定量限分别为0．1μg／mL和0．1μg／g（S／N≥10），血液中溴氰菊酯的回收率为91．55％～134．37％，日内精密度和日间精密度均小于5．67％。溴氰菊酯在512mg／kg剂量灌胃染毒大鼠体内分布为：肺〉肝〉心〉肾〉血液〉脑：在1024mg／kg剂量灌胃染毒大鼠体内分布为：肺〉血液〉心〉肾〉脑〉肝（P〈0．05）。结论本研究建立的溴氰菊酯GC—ECD检测方法灵敏度高。溴氰菊酯在体内的分布存在剂量依赖，血液、心、肝、肺、肾、脑可作为体内溴氰菊酯分析的良好检材。Objective To establish an animal model in acute poisoned rat by deltamethrin and an analy- sis method for determination of deltamethrin by gas chromatography-electron capture detector （GC-ECD） and to study the distribution of deltamethrin in rats in order to provide the references for forensic medicine identification about such cases. Methods Rats were administered with deltamethrin of different doses（512 and 1 024mg/kg） and killed 1.5 h later to be dissected rapidly for tissues （blood, hearts, livers, lungs, kidneys and brains etc.）. Samples were dehydrated by anhydrous sodium sulfate and extracted with petroleum ether and acetone （V：V=4：1）. The level of deltamethrin was determined by GC-ECD. Results There was a good separate between deltamethrin and endogenous impurities. The limit of quan- tification for deltamethrin in blood and liver were 0.1 txg/mL and 0.1 p.g/g （S/N~〉10）, respectively. The recovery rate of deltamethrin in blood was 91.55%-134.37% and both inter-day and intra-day precisions were less than 5.67%. The distribution of deltamethrin in poisoned rats with 512 mg/kg was as follow： lungs 〉 livers 〉 hearts 〉 kidneys 〉 blood 〉 brains and with 1 024 mg/kg dose was lungs 〉 blood 〉 hearts 〉 kid- neys 〉 brains 〉 livers （P〈0.05）. Conclusion The GC-ECD method is sensitive for determination of deltamethrin. The distribution of deltamethrin in rats has a dose-dependent manner. The study suggests that samples of blood, hearts, livers, lungs, kidneys and brains are suitable for deltamethrin poisoned analysis.

关 键 词：法医毒理学 中毒 溴氰菊酯 气相色谱-电子捕获检测器法 大鼠 

分 类 号：R979.8[医药卫生—药品]